艾灸对肠易激综合征小鼠行为学及色氨酸代谢相关产物的影响
艾灸对肠易激综合征小鼠行为学及色氨酸代谢相关产物的影响
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DOI：
10.1007/s11726-023-1366-4
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发表时间：
2023-04
影响因子：
0.5
通讯作者：
周次利
中科院分区：
文献类型：
--
作者：
陈嘉宜;朱笑吉;彭洋;刘慧荣;吴焕淦;周次利
观察艾灸对肠易激综合征（IBS）小鼠结肠色氨酸（Trp）代谢行为及相关产物的影响，探讨艾灸治疗IBS的机制。24只小鼠随机分为正常组、模型组、艾灸组和益生菌组，每组6只。采用2,4,6 - 三硝基苯磺酸（TNBS）溶液灌肠建立IBS内脏痛模型。艾灸组小鼠双侧足三里（ST36）温和灸，益生菌组小鼠采用双歧杆菌等益生菌灌胃。治疗后进行腹部撤回反射（AWR）试验、高架十字迷宫（EPM）试验和强迫游泳试验（FST）。免疫荧光法检测结肠5 - 羟色胺（5 - HT）和色氨酸羟化酶1（TPH1）的表达水平，酶联免疫吸附测定法检测结肠Trp、犬尿氨酸（Kyn）和吲哚 - 2,3 - 双加氧酶（IDO）的表达水平。与正常组相比，模型组在不同压力值下AWR评分显著升高（P<0.01），EPM试验中开臂停留时间和开臂进入次数显著减少（P<0.01，P<0.05），FST中静止时间显著增加（P<0.01），模型结肠Trp、TPH1、IDO、5 - HT和Kyn表达水平显著升高（P<0.01）。与模型组相比，艾灸组和益生菌组AWR评分有不同程度降低（P<0.05或P<0.01），EPM试验中开臂进入次数增加（P<0.05），FST中静止时间减少（P<0.05），结肠Trp、TPH1、IDO和5 - HT表达水平降低（P<0.01或P<0.05）；艾灸组开臂停留时间显著增加（P<0.01），益生菌组结肠Kyn表达水平显著降低（P<0.01）。足三里（ST36）艾灸可改善IBS小鼠内脏痛和疼痛情绪，下调结肠TPH1、IDO、Trp、5 - HT和Kyn的表达水平。
To observe the effect of moxibustion on behaviors and related products of tryptophan (Trp) metabolism in the colon of mice with irritable bowel syndrome (IBS), and to explore the mechanism of moxibustion in the IBS treatment. Twenty-four mice were randomly divided into a normal group, a model group, a moxibustion group, and a probiotic group, with 6 mice in each group. The visceral pain model of IBS was established by enema with 2,4,6-trinitrobenzene sulfonic acid (TNBS) solution. Mice in the moxibustion group were treated with mild moxibustion at bilateral Zusanli (ST36), and those in the probiotic group were treated with probiotics such as Bifidobacterium by gavage. Abdominal withdrawal reflex (AWR) test, elevated plus-maze (EPM) test, and forced swimming test (FST) were performed after treatment. The expression levels of 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase 1 (TPH1) in the colon were detected by immunofluorescence, and the expression levels of Trp, kynurenine (Kyn), and indole-2,3-oxygenase (IDO) in the colon were detected by enzyme-linked immunosorbent assay. Compared with the normal group, the AWR scores were increased significantly in the model group under different pressure values (P<0.01), the open-arm staying time and open-arm entries in the EPM test were decreased significantly (P<0.01, P<0.05), the motionless time in the FST was increased significantly (P<0.01), and the expression levels of colonic Trp, TPH1, IDO, 5-HT, and Kyn were increased significantly (P<0.01) in the models. Compared with the model group, the AWR scores were differently decreased (P<0.05 or P<0.01), the open-arm entries in the EPM test were increased (P<0.05), the motionless times in the FST were decreased (P<0.05), and the colonic expression levels of Trp, TPH1, IDO, and 5-HT were decreased (P<0.01 or P<0.05) in the moxibustion and probiotic groups; the open-arm staying time was significantly increased in the moxibustion group (P<0.01), and the colonic expression level of Kyn was significantly decreased in the probiotic group (P<0.01). Moxibustion at Zusanli (ST36) improves visceral pain and pain mood and down-regulates the expression levels of colonic TPH1, IDO, Trp, 5-HT, and Kyn in IBS mice.
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DOI：
10.1155/2015/361786
发表时间：
2015
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Shi Y;Chen YH;Yin XJ;Wang AQ;Chen XK;Lu JH;Ji R;Bao CH;Sun J;Zhao JM;Wu HG
通讯作者：
Wu HG
影响因子：
3.9
作者：
Gros M;Gros B;Mesonero JE;Latorre E
通讯作者：
Latorre E
影响因子：
4.3
作者：
Bao, Chun-Hui;Wang, Chun-Ye;Wu, Huan-Gan
通讯作者：
Wu, Huan-Gan
影响因子：
9.8
作者：
Cremon, Cesare;Carini, Giovanni;Barbara, Giovanni
通讯作者：
Barbara, Giovanni
影响因子：
9.8
作者：
Malinen, E;Rinttilä, T;Palva, A
通讯作者：
Palva, A