Zm908p11, encoded by a short open reading frame (sORF) gene, functions in pollen tube growth as a profilin ligand in maize.
Zm908p11, encoded by a short open reading frame (sORF) gene, functions in pollen tube growth as a profilin ligand in maize.
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Zm908p11 由短开放阅读框 (sORF) 基因编码，在玉米中作为 profilin 配体在花粉管生长中发挥作用。
DOI：
10.1093/jxb/ert093
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发表时间：
2013-05
影响因子：
6.9
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Dong X;Wang D;Liu P;Li C;Zhao Q;Zhu D;Yu J
开花植物的双受精作用依赖于精子通过花粉管定向运输到胚囊。目前，对玉米花粉萌发和花粉管生长的分子调控机制知之甚少。在这里，玉米花粉占主导地位的基因Zm 908，与几个推定的短开放阅读框架（sORF），分离和表征。Zm 908最长的ORF编码一个97个氨基酸的小蛋白。这被命名为Zm 908 p11，并分布在整个玉米花粉管中。Western blot检测到成熟花粉中的小肽。定量RT-PCR和北方杂交分析表明Zm 908 p11主要在成熟花粉粒中表达。在烟草中异位过表达全长Zm 908和Zm 908 p11导致有缺陷的花粉，而具有Zm 908 p11的位点特异性突变或移码突变的转基因烟草植株显示正常的花粉发育。Zm 908 p11在玉米中的过表达降低了花粉萌发效率。玉米花粉cDNA文库筛选和蛋白质相互作用分析表明，Zm 908 p11与玉米profilin 1（ZmPRO 1）相互作用。通过基因芯片分析，在Zm 908 p11转基因花粉中共检测到273个表达上调的基因和203个表达下调的基因。综上所述，这些结果表明Zm 908的功能与Zm 908 p11相同，并作为一种新的配体与profilins结合，在玉米花粉管生长过程中发挥所需的作用。因此，Zm 908 p11在玉米花粉管生长过程中的作用提出了一个模型。
Double fertilization of flowering plants depends on the targeted transportation of sperm to the embryo sac by the pollen tube. Currently, little is known about the underlying molecular mechanisms that regulate pollen germination and pollen tube growth in maize (Zea mays). Here, a maize pollen-predominant gene Zm908, with several putative short open reading frames (sORFs), was isolated and characterized. The longest ORF of Zm908 encodes a small protein of 97 amino acids. This was designated as Zm908p11 and is distributed throughout the maize pollen tube. Western blot detected the small peptide in mature pollen. Quantitative reverse transcription–PCR and northern blot analysis revealed that Zm908p11 was expressed predominantly in mature pollen grains. Ectopic overexpression of full-length Zm908 and Zm908p11 in tobacco resulted in defective pollen, while transgenic tobacco plants with a site-specific mutation or a frameshift mutation of Zm908p11 showed normal pollen development. Overexpression of Zm908p11 in maize decreased pollen germination efficiency. Maize pollen cDNA library screening and protein–protein interaction assays demonstrated that Zm908p11 interacts with maize profilin 1 (ZmPRO1). A microarray analysis identified 273 up-regulated and 203 down-regulated genes in the overexpressing transgenic Zm908p11 pollen. Taken together, these results indicate that Zm908 functions as Zm908p11, and binds to profilins as a novel ligand, with a required role during pollen tube growth in maize. Accordingly, a model is proposed for the role of Zm908p11 during pollen tube growth in maize.
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影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
DOI：
10.1073/pnas.91.18.8636
发表时间：
1994-08-30
影响因子：
11.1
作者：
FEDOROV, AA;MAGNUS, KA;ALMO, SC
通讯作者：
ALMO, SC
影响因子：
11.6
作者：
Gibbon, BC;Zonia, LE;Staiger, CJ
通讯作者：
Staiger, CJ
影响因子：
4
作者：
Ma, Jinxia;Yan, Bingxue;Ao, Guangming
通讯作者：
Ao, Guangming
DOI：
10.1038/10722
发表时间：
1999-07-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Mahoney, NM;Rozwarski, DA;Almo, SC
通讯作者：
Almo, SC