Intimal injury in vivo activates vascular smooth muscle cell migration and explant outgrowth in vitro.
Intimal injury in vivo activates vascular smooth muscle cell migration and explant outgrowth in vitro.
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体内内膜损伤激活血管平滑肌细胞迁移和体外外植体生长。
DOI：
10.1161/01.atv.4.3.183
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Haudenschild,CC
中科院分区：
文献类型：
--
作者：
Grunwald,J;Haudenschild,CC
体内诱导的内皮损伤导致内膜增厚，这主要是由平滑肌细胞（SMC）的刺激引起的，平滑肌细胞迁移到剥脱的内膜中并在剥脱的内膜中增殖。我们评估了这种刺激的表现和持久性，在几个体外外植体和生长测定使用主动脉外植体从球囊导管损伤的大鼠或假手术对照。对照外植体的SMC生长在移植后48小时开始，96小时后达到半最大值，74%的外植体显示出生长。球囊损伤的腹主动脉的外植体在24小时内表现出SMC生长，48小时后达到一半最大值，92%的外植体表现出生长。球囊损伤和处死之间的间隔为4至7天是获得加速生长的最佳时间，而1天的间隔显示出弱刺激，21天的间隔没有这种效果。体内刺激的细胞生长更快，导致每个外植体的菌落大小更大，并且它们暂时不依赖血清。生长速率增加持续到第二次传代，但在随后的传代中恢复正常。SMC迁移的机制现在可以通过用组织培养皿代替内膜来定量评估，从而可以研究致动脉粥样硬化刺激的程度和持久性。
In vivo induced endothelial injury results in an intimal thickening, mainly caused by stimulation of smooth muscle cells (SMC), which migrate into, and proliferate in, the denuded intima. We evaluated the manifestation and persistence of this stimulus in several in vitro explant and growth assays using aortic explants from either balloon catheter-injured rats or from sham-operated controls. SMC outgrowth from control explants started 48 hours after explantation, became half-maximal after 96 hours, with 74% of the explants showing outgrowth. Explants from balloon-injured aortas showed SMC outgrowth within 24 hours, became half-maximal after 48 hours, with 92% of the explants showing outgrowth. An interval of 4 to 7 days between balloon injury and sacrifice was optimal for obtaining accelerated outgrowth, while a 1-day interval showed a weak stimulation and a 21-day interval had no such effect. The in vivo stimulated cells grew more rapidly, resulting in a greater colony size per explant, and they became temporarily serum-independent. Increased growth rate persisted up to the second subculture but returned to normal in subsequent passages. Mechanisms of SMC migration can now be quantitatively assessed by replacing the intima with the tissue culture dish, allowing studies on the extent and persistence of atherogenic stimuli.
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实验性动脉硬化
影响因子：
15.3
作者：
M. Stemerman;R. Ross
通讯作者：
R. Ross
灵长类动物动脉中的蛋白多糖。
影响因子：
7.8
作者：
T. Wight;R. Ross
通讯作者：
R. Ross
培养的动脉粥样硬化兔主动脉平滑肌细胞增强胶原蛋白和总蛋白的合成。
影响因子：
5.3
作者：
K. Pietilä;T. Nikkari
通讯作者：
T. Nikkari
兔主动脉内膜损伤和再生：内侧平滑肌细胞作为新内膜的来源
影响因子：
20.1
作者：
T. Spaet;M. Stemerman;F. Veith;I. Lejnieks
通讯作者：
I. Lejnieks
从兔主动脉去除内皮的延迟后果。
影响因子：
--
作者：
T. Spaet;C. Rhee;C. Geiger
通讯作者：
C. Geiger