Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization.
Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis: its cellular distribution, thioredoxin activity and role in heme utilization.
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DOI：
10.1186/1471-2180-10-152
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发表时间：
2010-05-25
期刊：
影响因子：
4.2
通讯作者：
Nakayama K
中科院分区：
文献类型：
--
作者：
Shoji M;Shibata Y;Shiroza T;Yukitake H;Peng B;Chen YY;Sato K;Naito M;Abiko Y;Reynolds EC;Nakayama K
牙周病原体牙龈卟啉单胞菌是一种专性厌氧菌，需要血红素生长。为了了解其血红素的获取机制，我们集中在血红素结合蛋白（HBP 35蛋白），具有一个硫氧还蛋白样基序和一个保守的C-末端结构域，这被认为是参与氧化还原调节和细胞表面附着，分别。我们观察到，hbp 35基因被转录为一个1.1 kb的mRNA与随后的翻译产生三个蛋白质的分子量为40，29和27 kDa的细胞质中，和一个修改后的形式的40 kDa的蛋白质的细胞表面上。重组40-kDa HBP 35在体外表现出硫氧还蛋白活性，两个假定的活性位点半胱氨酸残基的突变废除了这种活性。重组40-和27-kDa的蛋白质有能力结合氯化血红素，和生长的hbp 35缺失突变体的氯化血红素耗尽的条件下相比，在相同的条件下的野生型的生长基本上被推迟。牙龈卟啉单胞菌HBP 35表现出硫氧还蛋白和氯化血红素结合活性，并且对于氯化血红素耗尽条件下的生长是必需的，这表明该蛋白质在氯化血红素获得中起重要作用。
The periodontal pathogen Porphyromonas gingivalis is an obligate anaerobe that requires heme for growth. To understand its heme acquisition mechanism, we focused on a hemin-binding protein (HBP35 protein), possessing one thioredoxin-like motif and a conserved C-terminal domain, which are proposed to be involved in redox regulation and cell surface attachment, respectively. We observed that the hbp35 gene was transcribed as a 1.1-kb mRNA with subsequent translation resulting in three proteins with molecular masses of 40, 29 and 27 kDa in the cytoplasm, and one modified form of the 40-kDa protein on the cell surface. A recombinant 40-kDa HBP35 exhibited thioredoxin activity in vitro and mutation of the two putative active site cysteine residues abolished this activity. Both recombinant 40- and 27-kDa proteins had the ability to bind hemin, and growth of an hbp35 deletion mutant was substantially retarded under hemin-depleted conditions compared with growth of the wild type under the same conditions. P. gingivalis HBP35 exhibits thioredoxin and hemin-binding activities and is essential for growth in hemin-depleted conditions suggesting that the protein plays a significant role in hemin acquisition.
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影响因子：
2.1
作者：
Hiratsuka, K;Yoshida, W;Abiko, Y
通讯作者：
Abiko, Y
DOI：
10.1016/s0306-3623(96)00366-7
发表时间：
1997-05-01
期刊：
GENERAL PHARMACOLOGY
影响因子：
--
作者：
Saito, S;Hiratsuka, K;Abiko, Y
通讯作者：
Abiko, Y
影响因子：
3.2
作者：
Dashper, Stuart G.;Ang, Ching-Seng;Reynolds, Eric C.
通讯作者：
Reynolds, Eric C.
影响因子：
3.2
作者：
Nelson, KE;Fleischmann, RD;Fraser, CM
通讯作者：
Fraser, CM
影响因子：
3.2
作者：
Rocha, Edson R.;Tzianabos, Arthur O.;Smith, C. Jeffrey
通讯作者：
Smith, C. Jeffrey