Functional identification of Proteus mirabilis eptC gene encoding a core lipopolysaccharide phosphoethanolamine transferase.
Functional identification of Proteus mirabilis eptC gene encoding a core lipopolysaccharide phosphoethanolamine transferase.
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DOI：
10.3390/ijms15046689
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发表时间：
2014-04-21
影响因子：
5.6
通讯作者：
Tomás JM
中科院分区：
文献类型：
--
作者：
Aquilini E;Merino S;Knirel YA;Regué M;Tomás JM
通过将奇异变形杆菌HI4320基因组与已知的脂多糖磷酸乙醇胺转移酶进行比较，确定了3个可能的候选基因(PMI3040、PMI3576和PMI3104)。其中，eptC(PMI3104)能够修饰肺炎克雷伯菌两个已定义的非极性核心脂多糖突变体的脂多糖，我们将其用作替代底物。质谱学和核磁共振结果表明，eptC基因直接将磷酸乙醇胺掺入到L-甘油-d-甘露-庚糖II的O-6位，在本研究所分析的所有奇异拟青霉中都存在eptC基因。仅在肠杆菌科家族的另外两个属中发现了推测的eptC同源物，发光菌和普罗维登西亚。本研究结果支持奇异P.mirabilis菌株中eptC(PMI3104)产物在将PETN转移到L，d-HepII的O-6位上的作用。
By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains.
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影响因子：
3.2
作者：
Izquierdo, L;Coderch, N;Regué, M
通讯作者：
Regué, M
假定变形杆菌 O 抗原基因座的分子和遗传学分析
影响因子：
4.4
作者：
Wang, Quan;Torzewska, Agnieszka;Wang, Lei
通讯作者：
Wang, Lei
影响因子：
3.2
作者：
Wenzel, Cory Q.;St Michael, Frank;Richards, James C.
通讯作者：
Richards, James C.
影响因子：
3.2
作者：
HITCHCOCK, PJ;BROWN, TM
通讯作者：
BROWN, TM
影响因子：
3
作者：
Himpsl, Stephanie D.;Lockatell, C. Virginia;Mobley, Harry L. T.
通讯作者：
Mobley, Harry L. T.