Lack of Increase in Muscle Mitochondrial Protein Synthesis During the Course of Aerobic Exercise and Its Recovery in the Fasting State Irrespective of Obesity.
Lack of Increase in Muscle Mitochondrial Protein Synthesis During the Course of Aerobic Exercise and Its Recovery in the Fasting State Irrespective of Obesity.
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DOI：
10.3389/fphys.2021.702742
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发表时间：
2021
影响因子：
4
通讯作者：
Katsanos CS
中科院分区：
文献类型：
--
作者：
Serrano N;Tran L;Hoffman N;Roust L;De Filippis EA;Carroll CC;Patel SH;Kras KA;Buras M;Katsanos CS
急性有氧运动会诱导骨骼肌线粒体基因表达，进而增加肌肉线粒体蛋白质合成。在这方面，过氧化物酶体增殖物激活受体 γ 共激活剂 1α (PGC-1α) 是线粒体生物发生以及线粒体蛋白质合成的主要调节因子。然而，肥胖人群的肌肉中 PGC-1α 表达因急性有氧运动而受损。因此，我们试图确定肥胖人群在相同条件下肌肉线粒体蛋白质合成是否也受到损害。为此，我们测量了未经训练的肥胖（体脂：34.7 ± 2.3%）和无肥胖（体脂：25.3 ± 3.3%）受试者的骨骼肌中线粒体和混合肌蛋白合成的情况，这些受试者在基础期和连续期内，包括 45 分钟自行车运动（以对应于心率储备的 65% 的强度进行）和 3 小时运动后恢复。运动会增加非肥胖受试者肌肉中 PGC-1α mRNA 的表达，但不会增加肥胖受试者的肌肉中 PGC-1α mRNA 的表达。然而，两组受试者的肌肉线粒体蛋白质合成均没有增加。同样，两组的混合肌肉蛋白质合成都没有增加。在非肥胖受试者中，血浆氨基酸浓度在运动后降低，但在肥胖受试者中则没有。我们得出的结论是，无论是否肥胖，在一次有氧运动过程中以及在禁食状态下的恢复期间，人类肌肉中的线粒体和混合肌肉蛋白质合成都不会增加。试验注册：该研究已在 ClinicalTrials.gov 内注册 (NCT01824173)。
Acute aerobic exercise induces skeletal muscle mitochondrial gene expression, which in turn can increase muscle mitochondrial protein synthesis. In this regard, the peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), is a master regulator of mitochondrial biogenesis, and thus mitochondrial protein synthesis. However, PGC-1α expression is impaired in muscle of humans with obesity in response to acute aerobic exercise. Therefore, we sought to determine whether muscle mitochondrial protein synthesis is also impaired under the same conditions in humans with obesity. To this end, we measured mitochondrial and mixed-muscle protein synthesis in skeletal muscle of untrained subjects with (body fat: 34.7 ± 2.3%) and without (body fat: 25.3 ± 3.3%) obesity in a basal period and during a continuous period that included a 45 min cycling exercise (performed at an intensity corresponding to 65% of heart rate reserve) and a 3-h post-exercise recovery. Exercise increased PGC-1α mRNA expression in muscle of subjects without obesity, but not in subjects with obesity. However, muscle mitochondrial protein synthesis did not increase in either subject group. Similarly, mixed-muscle protein synthesis did not increase in either group. Concentrations of plasma amino acids decreased post-exercise in the subjects without obesity, but not in the subjects with obesity. We conclude that neither mitochondrial nor mixed-muscle protein synthesis increase in muscle of humans during the course of a session of aerobic exercise and its recovery period in the fasting state irrespective of obesity. Trial Registration: The study has been registered within ClinicalTrials.gov (NCT01824173).
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DOI：
10.1097/mco.0b013e3283412343
发表时间：
2011-01
影响因子：
3.1
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Markofski MM;Volpi E
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影响因子：
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2003-03-01
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DOI：
10.1152/ajpendo.00393.2004
发表时间：
2005-03-01
影响因子：
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