Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method.
Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method.
复制标题
基于金纳米粒子适配体的局域表面等离子体共振 (LSPR) 传感，采用双校准曲线法扩大检测范围的赭曲霉毒素 A
DOI：
10.3389/fchem.2018.00094
复制
发表时间：
2018
影响因子：
5.5
通讯作者：
He Z
中科院分区：
文献类型：
--
作者：
Liu B;Huang R;Yu Y;Su R;Qi W;He Z
赭曲霉毒素A(OTA)是曲霉菌和青霉菌代谢产生的一种真菌毒素，对人、畜、禽都有极强的毒性。然而，传统的检测OTA的方法昂贵且复杂。除了OTA适配子外，高浓度的OTA本身也可以吸附在金纳米颗粒(AuNPs)的表面，进一步抑制AuNPs的盐团聚。在此，我们报告了一种新的OTA分析方法，它利用金纳米颗粒及其聚集体的局域表面等离子体共振效应。针对单一线性校正曲线测量结果不可靠的问题，提出了一种双校正曲线的方法来解决这一问题，扩大了OTA检测范围。还检测了一些其他分析物，并进一步讨论了与AuNPs结合的分析物的结构性质。我们发现，由于这些分析物的结合强度不同，在应用基于AuNP聚集的方法时，必须考虑到各种因素。
Ochratoxin A (OTA) is a type of mycotoxin generated from the metabolism of Aspergillus and Penicillium, and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentration can also adsorb on the surface of gold nanoparticles (AuNPs), and further inhibit AuNPs salt aggregation. We herein report a new OTA assay by applying the localized surface plasmon resonance effect of AuNPs and their aggregates. The result obtained from only one single linear calibration curve is not reliable, and so we developed a “double calibration curve” method to address this issue and widen the OTA detection range. A number of other analytes were also examined, and the structural properties of analytes that bind with the AuNPs were further discussed. We found that various considerations must be taken into account in the detection of these analytes when applying AuNP aggregation-based methods due to their different binding strengths.
登录
查看更多内容
影响因子：
4.2
作者：
Malir F;Ostry V;Pfohl-Leszkowicz A;Malir J;Toman J
通讯作者：
Toman J
影响因子：
3.9
作者：
Kumar, A;Mandal, S;Sastry, M
通讯作者：
Sastry, M
影响因子：
4.9
作者：
Maddanimath, T;Kumar, A;Ramanath, G
通讯作者：
Ramanath, G
影响因子：
5.5
作者：
Fiore E;Dausse E;Dubouchaud H;Peyrin E;Ravelet C
通讯作者：
Ravelet C
影响因子：
6.1
作者：
Cruz-Aguado, Jorge A.;Penner, Gregory
通讯作者：
Penner, Gregory