Comparative transcriptomic analysis of maize ear heterosis during the inflorescence meristem differentiation stage.
Comparative transcriptomic analysis of maize ear heterosis during the inflorescence meristem differentiation stage.
复制标题
花序分生组织分化阶段玉米穗杂种优势的比较转录组分析。
DOI：
10.1186/s12870-022-03695-6
复制
发表时间：
2022-07-18
影响因子：
5.3
通讯作者：
Tang, Jihua
中科院分区：
文献类型：
--
作者：
Shi, Xia;Li, Weihua;Guo, Zhanyong;Wu, Mingbo;Zhang, Xiangge;Yuan, Liang;Qiu, Xiaoqian;Xing, Ye;Sun, Xiaojing;Xie, Huiling;Tang, Jihua
关键词：
杂种优势在许多作物中得到广泛应用，对全球粮食安全具有重要意义，而玉米是利用杂种优势最成功的作物之一。基因表达模式控制玉米穗的发育，但杂种优势影响转录水平控制的机制尚未完全了解。本研究以含有穗宽相关杂种优势位点hlEW 2b的单片段代换系lx 9801 hlEW 2b、受体亲本lx 9801、测试亲本郑58及其相应的杂交种郑58 × lx 9801 hlEW 2b（HY）和郑58 × lx 9801（CK）为材料，对玉米穗部花序分生组织进行了取样分析。经RNA测序和转录组学分析，2531个独特的差异表达基因（DEG）之间的两个杂种（HY与CK）进行了鉴定。我们的研究结果表明，大约64%和48%的DEG在HY和CK中表现出加性表达，而其他基因则表现出非加性表达模式。DEGs在多代谢过程、植物器官形态建成和激素调节的GO功能类别中显著富集。这些基本过程可能与玉米穗发育期的杂种优势表现有关。特别是，125和100 DEG的杂交种等位基因特异性表达（ASE），分别在HY和CK中进行了特异性鉴定。两个杂种的比较表明，ASE基因参与不同的发育相关过程，可能导致玉米穗发育过程中的杂种优势表型。此外，几个关键基因参与生长素代谢和IM发展的杂交种之间的差异表达，并表现出不同的表达模式（加性，非加性，和ASE）。这些基因表达水平的变化可能导致IM中生长素稳态的差异，影响控制IM发育的核心基因如WUS的转录。我们的研究表明，加性、非加性和等位基因特异性表达模式可以微调调节碳水化合物和蛋白质代谢过程、氮同化和生长素代谢的关键DEG的表达至最佳水平，这些转录变化可能在玉米穗杂种优势中发挥重要作用。这些结果为深入了解玉米穗发育过程中转录水平的变化与杂种优势的关系提供了新的信息，有助于阐明杂种优势的遗传和分子机制。在线版本包含补充材料，可通过10.1186/s12870-022-03695-6获得。
Heterosis is widely used in many crops and is important for global food safety, and maize is one of the most successful crops to take advantage of heterosis. Gene expression patterns control the development of the maize ear, but the mechanisms by which heterosis affects transcriptional-level control are not fully understood. In this study, we sampled ear inflorescence meristems (IMs) from the single-segment substitution maize (Zea mays) line lx9801hlEW2b, which contains the heterotic locus hlEW2b associated with ear width, as well as the receptor parent lx9801, the test parent Zheng58, and their corresponding hybrids Zheng58 × lx9801hlEW2b (HY) and Zheng58 × lx9801 (CK). After RNA sequencing and transcriptomic analysis, 2531 unique differentially expressed genes (DEGs) were identified between the two hybrids (HY vs. CK). Our results showed that approximately 64% and 48% of DEGs exhibited additive expression in HY and CK, whereas the other genes displayed a non-additive expression pattern. The DEGs were significantly enriched in GO functional categories of multiple metabolic processes, plant organ morphogenesis, and hormone regulation. These essential processes are potentially associated with heterosis performance during the maize ear developmental stage. In particular, 125 and 100 DEGs from hybrids with allele-specific expression (ASE) were specifically identified in HY and CK, respectively. Comparison between the two hybrids suggested that ASE genes were involved in different development-related processes that may lead to the hybrid vigor phenotype during maize ear development. In addition, several critical genes involved in auxin metabolism and IM development were differentially expressed between the hybrids and showed various expression patterns (additive, non-additive, and ASE). Changes in the expression levels of these genes may lead to differences in auxin homeostasis in the IM, affecting the transcription of core genes such as WUS that control IM development. Our research suggests that additive, non-additive, and allele-specific expression patterns may fine-tune the expression of crucial DEGs that modulate carbohydrate and protein metabolic processes, nitrogen assimilation, and auxin metabolism to optimal levels, and these transcriptional changes may play important roles in maize ear heterosis. The results provide new information that increases our understanding of the relationship between transcriptional variation and heterosis during maize ear development, which may be helpful for clarifying the genetic and molecular mechanisms of heterosis. The online version contains supplementary material available at 10.1186/s12870-022-03695-6.
登录
查看更多内容
影响因子：
5.4
作者：
Guo, Mei;Rupe, Mary A.;Bowen, Ben
通讯作者：
Bowen, Ben
影响因子：
5.1
作者：
Huang, Yi;Zhang, Lida;Zhang, Qifa
通讯作者：
Zhang, Qifa
影响因子：
16.6
作者：
Jia, Haitao;Li, Manfei;Zhang, Zuxin
通讯作者：
Zhang, Zuxin
影响因子：
12.3
作者：
Krecek P;Skupa P;Libus J;Naramoto S;Tejos R;Friml J;Zazímalová E
通讯作者：
Zazímalová E
影响因子：
3.3
作者：
Hoecker, Nadine;Keller, Barbara;Hochholdinger, Frank
通讯作者：
Hochholdinger, Frank