Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells
Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells
复制标题
视黄酸诱导小鼠胚胎癌细胞分化过程中出现核纤层蛋白 A 和 C
作者：
S. Lebel;C. Lampron;A. Royal;Y. Raymond
在视黄酸诱导的培养分化过程中研究了从 F9 胚胎癌细胞中分离出的核基质的核纤层蛋白补体。原始细胞向壁内胚层样细胞的分化伴随着核纤层蛋白A和C的逐渐出现，而核纤层蛋白B贯穿所有阶段。通过分子质量、等电点、单克隆抗体和多克隆抗血清的识别以及肽图谱来鉴定核纤层蛋白。基质中发现的核纤层蛋白 A 和 C 数量的增加是由于从头合成，因为在未分化细胞中没有检测到这些核纤层蛋白的核外池。这些结果提供了生化证据，表明与两栖动物胚胎发生一样，哺乳动物发育过程中核纤层组成存在变化。
The lamin complement of nuclear matrix isolated from F9 embryonal carcinoma cells was studied during retinoic acid-induced differentiation in culture. Differentiation of the original cells into parietal endoderm-like cells was accompanied by the gradual appearance of lamins A and C while lamin B was present throughout all stages. Lamins were identified by their molecular masses, isoelectric points, recognition by a monoclonal antibody and a polyclonal antiserum, and by peptide mapping. The increase in the amounts of lamins A and C found in the matrix was due to de novo synthesis as no extranuclear pools of these lamins were detected in the undifferentiated cells. These results provide biochemical evidence that, as in amphibian embryogenesis, there are variations in nuclear lamina composition during mammalian development.
一种在十二烷基硫酸钠-聚丙烯酰胺凝胶中使用 N-氯代琥珀酰亚胺/尿素和肽银染色进行部分肽图谱分析的新方法。
影响因子：
2.9
作者：
Lischwe,MA;Ochs,D
通讯作者：
Ochs,D
DOI：
10.1073/pnas.82.14.4727
发表时间：
1985-01-01
影响因子：
11.1
作者：
SCHATTEN, G;MAUL, GG;BROWN, DL
通讯作者：
BROWN, DL
小鼠精子发生核分化过程中核纤层蛋白的识别和重新分布。
影响因子：
2.7
作者：
Maul,GG;French,BT;Bechtol,KB
通讯作者：
Bechtol,KB