Differential effects of naloxone on the release of neurohypophysial hormones in normotensive and spontaneously hypertensive rats.
Differential effects of naloxone on the release of neurohypophysial hormones in normotensive and spontaneously hypertensive rats.
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纳洛酮对正常血压和自发性高血压大鼠神经垂体激素释放的不同影响。
DOI：
10.1016/0006-8993(85)90317-8
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Summy-Long,JY
中科院分区：
文献类型：
--
作者：
Rosella-Dampman,LM;Emmert,SE;Keil,LC;Summy-Long,JY
自发性高血压大鼠（SHR）的下丘脑-神经垂体系统发生改变。我们假设内源性阿片肽对抗利尿激素（VP）和催产素（OT）释放的异常调节改变了SHR中的神经内分泌系统。测定17周龄SHRs大鼠和2株正常血压对照Wistar Kyoto （WKY）和Sprague-Dawley大鼠血浆和垂体神经垂体激素的浓度。在皮下注射生理盐水（1 ml/kg）或盐酸纳洛酮（1或10 mg/kg） 20分钟后，将动物斩首。此外，测定代谢笼饲养5 d的16周龄大鼠在白天（08.00-17.30 h）和夜间（17.30-08.00 h）尿液中分泌的神经垂体激素。下丘脑外部位的VP也在皮质下器官区、海马交穹窿和脉络膜丛的酸提取物中被测量为[VP]。用放射免疫法测定激素含量。高血压动物hrs垂体含量、血浆浓度和尿中OT含量降低（P < 0.05），而高血压动物垂体和血浆VP含量升高（P < 0.05），尿中VP含量不变。下丘脑外组织中，海马交穹窿[VP]在SHR中升高。纳洛酮可显著提高WKY动物血浆OT和SHRs动物血浆VP浓度（P < 0.05）。纳洛酮对Sprague-Dawley大鼠血浆[VP]和[OT]均无影响。纳洛酮对高血压和正常血压大鼠脑垂体神经垂体激素的储存均无影响。综上所述，在16-17周龄的大鼠中，内源性阿片肽能抑制OT的释放，而在SHRs中，阿片肽能降低VP的释放。阿片肽在神经垂体激素释放中的神经调节作用似乎在SHR中被改变，从而抑制VP释放和增强OT释放。
The hypothalamo-neurohypophysial system is altered in the spontaneously hypertensive rat (SHR). We hypothesized that an aberrant regulation of vasopressin (VP) and oxytocin (OT) release by endogenous opiod peptides alters this neuroendocrine system in the SHR. Concentrations of the neurohypophysial hormones in plasma and the pituitary were measured in 17-week-old SHRs and two strains of normotensive controls, Wistar Kyoto (WKY) and Sprague-Dawley rats. Animals were decapitated 20 min after s.c. injection of saline (1 ml/kg) or naloxone hydrochloride (1 or 10 mg/kg). In addition, neurohypophysial hormones excreted during the day (08.00–17.30 h) and night (17.30–08.00 h) were determined in urine from 16-week-old animals kept in metabolic cages for 5 days. VP at extrahypothalamic sites was also measured as [VP] in acid extracts of the subfornical organ area, hippocampal commissure-fornix and choriod plexus. Hormones were quantified by radioimmunoassay. The pituitary content, plasma concentration, and urinary excretion of OT were reduced (P < 0.05) in SHRs, whereas VP content was increased (P < 0.05) in the pituitary and plasma, but unchanged in urine, of hypertensive animals. In extrahypothalamic tissues, [VP] in the hippocampal commissure-fornix was increased in the SHR. Naloxone elevated (P < 0.05) the plasma concentration of OT in WKY animals and VP in SHRs. Neither [VP] nor [OT] in plasma was changed by naloxone in Sprague-Dawley rats. Pituitary stores of the neurohypophysial hormones were not altered by naloxone in either hypertensive or normotensive rats. In conclusion, endogenous opioid peptides tonically inhibit OT release in WKY rats, whereas VP releas is decreased by opioid peptides in SHRs, 16–17 weeks of age. The neuromodulatory role of opioid peptides in the release of neurohypophysial hormones appears to be altered in the SHR such that VP release is suppressed and OT release is augmented.
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