Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii.
Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii.
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DOI：
10.1186/1471-2229-7-45
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发表时间：
2007-08-22
影响因子：
5.3
通讯作者：
Krause K
中科院分区：
文献类型：
--
作者：
Funk HT;Berg S;Krupinska K;Maier UG;Krause K
全寄生植物菟丝子属（Cuscuta）包括具有光合能力和功能性叶绿体的物种以及具有受限光合活性和退化叶绿体的无叶绿素和中间形式。先前的数据表明，在不同的菟丝子种，可能与他们的光合活性的质体基因组编码能力的显着差异。为了阐明伴随寄生生活方式的分子变化，我们对两种菟丝子（Cuscuta reflexa和Cuscutagronovii）的质体染色体进行了测序。这两个物种都能够进行光合作用，尽管效率不同。与Epifagus virginiana的质体基因组一起，Epifagus virginiana是一种无叶绿素的寄生植物，其质体基因组已被测序，这些物种代表了一系列对宿主植物完全依赖的进展，从C.反折导致光合活性降低和叶绿体退化。gronovii转化为无叶绿素状态。弗吉尼亚。最新测定的C. reflexa和C. Gronovii的研究表明，尽管鉴定出许多种特异性插入、缺失（indels）和序列倒位，但染色体结构通常与非寄生植物的非常相似。然而，我们观察到质体基因组逐渐适应不同程度的寄生。这种变化在C. gronovii的突变，包括（a）质体编码的RNA聚合酶亚基和相应启动子的基因从质体基因组中平行丢失，（B）推定剪接因子MatK的基因从质体基因组中首次记录丢失，和（c）RNA编辑的显著减少。总体而言，从寄生植物质体DNA的比较基因组分析表明，作为对宿主植物的依赖性增加的结果，对质体基因表达机制的简化的偏见。从目前的数据集可以推断出一个试探性的分配连续事件的适应质体基因组寄生。这包括（1）在具有光合作用的菟丝子属物种中非编码区的丢失，这导致质体基因组的浓缩，（2）在具有严重受损的光合能力的物种中质体基因表达的简化，以及（3）在非光合作用寄生植物中，遗传信息的重要部分的缺失，包括光合机构的信息。
The holoparasitic plant genus Cuscuta comprises species with photosynthetic capacity and functional chloroplasts as well as achlorophyllous and intermediate forms with restricted photosynthetic activity and degenerated chloroplasts. Previous data indicated significant differences with respect to the plastid genome coding capacity in different Cuscuta species that could correlate with their photosynthetic activity. In order to shed light on the molecular changes accompanying the parasitic lifestyle, we sequenced the plastid chromosomes of the two species Cuscuta reflexa and Cuscuta gronovii. Both species are capable of performing photosynthesis, albeit with varying efficiencies. Together with the plastid genome of Epifagus virginiana, an achlorophyllous parasitic plant whose plastid genome has been sequenced, these species represent a series of progression towards total dependency on the host plant, ranging from reduced levels of photosynthesis in C. reflexa to a restricted photosynthetic activity and degenerated chloroplasts in C. gronovii to an achlorophyllous state in E. virginiana. The newly sequenced plastid genomes of C. reflexa and C. gronovii reveal that the chromosome structures are generally very similar to that of non-parasitic plants, although a number of species-specific insertions, deletions (indels) and sequence inversions were identified. However, we observed a gradual adaptation of the plastid genome to the different degrees of parasitism. The changes are particularly evident in C. gronovii and include (a) the parallel losses of genes for the subunits of the plastid-encoded RNA polymerase and the corresponding promoters from the plastid genome, (b) the first documented loss of the gene for a putative splicing factor, MatK, from the plastid genome and (c) a significant reduction of RNA editing. Overall, the comparative genomic analysis of plastid DNA from parasitic plants indicates a bias towards a simplification of the plastid gene expression machinery as a consequence of an increasing dependency on the host plant. A tentative assignment of the successive events in the adaptation of the plastid genomes to parasitism can be inferred from the current data set. This includes (1) a loss of non-coding regions in photosynthetic Cuscuta species that has resulted in a condensation of the plastid genome, (2) the simplification of plastid gene expression in species with largely impaired photosynthetic capacity and (3) the deletion of a significant part of the genetic information, including the information for the photosynthetic apparatus, in non-photosynthetic parasitic plants.
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DOI：
10.1098/rstb.2000.0697
发表时间：
2000-10-29
影响因子：
6.3
作者：
Allen, JF;Pfannschmidt, T
通讯作者：
Pfannschmidt, T
影响因子：
11.4
作者：
Hajdukiewicz, PTJ;Allison, LA;Maliga, P
通讯作者：
Maliga, P
DOI：
10.1007/bf00299148
发表时间：
1992-03-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
HABERHAUSEN, G;VALENTIN, K;ZETSCHE, K
通讯作者：
ZETSCHE, K
影响因子：
2.5
作者：
KAPOOR, S;WAKASUGI, T;SUGIURA, M
通讯作者：
SUGIURA, M
影响因子：
4.3
作者：
Berg, S;Krause, K;Krupinska, K
通讯作者：
Krupinska, K