Exosomal vaccines containing the S protein of the SARS coronavirus induce high levels of neutralizing antibodies.
Exosomal vaccines containing the S protein of the SARS coronavirus induce high levels of neutralizing antibodies.
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DOI：
10.1016/j.virol.2006.12.011
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发表时间：
2007-05-25
期刊：
影响因子：
3.7
通讯作者：
Uberla K
中科院分区：
文献类型：
--
作者：
Kuate S;Cinatl J;Doerr HW;Uberla K
感染SARS冠状病毒(SARS-CoV)会导致一种致死率很高的非典型肺部疾病。尽管最初的SARS疫情得到了控制，但仍有零星的疾病暴发，这表明持续需要针对这种病毒的疫苗。因此，我们探索了含有SARS-CoV尖峰S蛋白的外体疫苗。将S蛋白的跨膜区和胞浆区替换为VSv-G，获得了含有S的外切体。在小鼠身上测试了含有S的外切体的免疫原性和效力，并与表达S蛋白的腺病毒载体疫苗进行了比较。S外切体和腺病毒载体疫苗均可诱导中和抗体滴度。用SARS-S胞外体疫苗免疫和腺病毒载体加强免疫后，中和抗体效价超过1例SARS患者恢复期血清中和抗体滴度。这两种方法在表达SARS-S的肿瘤挑战模型中都是有效的，因此值得进一步研究。
Infection with the SARS-associated coronavirus (SARS-CoV) induces an atypical pulmonary disease with a high lethality rate. Although the initial SARS epidemic was contained, sporadic outbreaks of the disease still occur, suggesting a continuous need for a vaccine against this virus. We therefore explored exosome-based vaccines containing the spike S proteins of SARS-CoV. S-containing exosomes were obtained by replacing the transmembrane and cytoplasmic domains of the S protein by those of VSV-G. The immunogenicity and efficacy of the S-containing exosomes were tested in mice and compared to an adenoviral vector vaccine expressing the S protein. Both, S-containing exosomes and the adenoviral vector vaccine induced neutralizing antibody titers. After priming with the SARS-S exosomal vaccine and boosting with the adenoviral vector the neutralizing antibody titers exceeded those observed in the convalescent serum of a SARS patient. Both approaches were effective in a SARS-S-expressing tumor challenge model and thus warrant further investigation.
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