Gene expression of O-GlcNAc cycling enzymes in human breast cancers.
Gene expression of O-GlcNAc cycling enzymes in human breast cancers.
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DOI：
10.1007/s10238-011-0138-5
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发表时间：
2012-03
影响因子：
4.6
通讯作者：
Brys, Magdalena
中科院分区：
文献类型：
--
作者：
Krzeslak, Anna;Forma, Ewa;Bernaciak, Magdalena;Romanowicz, Hanna;Brys, Magdalena
o - glcn酰化是一种丰富的、动态的、可诱导的翻译后修饰，其中单个β- n -乙酰氨基葡萄糖胺残基通过o -糖苷键连接到丝氨酸或tre氨酸残基上。提示异常调节的o - glcn酰化可能与肿瘤病理有关。O-GlcNAc残基在细胞内蛋白上的循环由两种酶控制，一种是O-GlcNAc转移酶（OGT），它催化O-GlcNAc残基的添加，另一种是核质β- n -乙酰氨基葡萄糖酶（O-GlcNAcase，由MGEA5基因编码），参与O-GlcNAc的去除。本研究分析了乳腺导管癌中O-GlcNAc循环酶编码基因mRNA表达与临床病理参数的关系。结果显示，低分化肿瘤（II级和III级）的OGT表达明显高于I级肿瘤。相反，与I级肿瘤相比，II级和III级肿瘤的MGEA5转录水平明显较低。Spearman秩相关分析显示，OGT与MGEA5在乳腺癌中的表达呈负相关（r = - 0.430, P = 0.0002）。淋巴结转移状态与mgea5mrna表达降低显著相关。这一结果表明，O-GlcNAc修饰蛋白的升高可能与乳腺肿瘤的进展和转移有关。
O-GlcNAcylation is an abundant, dynamic, and inducible posttranslational modification in which single β-N-acetylglucosamine residues are attached by O-glycosidic linkage to serine or treonine residues. It is suggested that abnormally regulated O-GlcNAcylation may contribute to the pathology of cancer. Cycling of O-GlcNAc residues on intracellular proteins is controlled by two enzymes, O-GlcNAc transferease (OGT), which catalyses the addition of O-GlcNAc residues and nucleocytoplasmic β-N-acetylglucosaminidase (O-GlcNAcase; encoded by MGEA5 gene), an enzyme involved in the removal of O-GlcNAc. In this study, relationship between the mRNA expressions of genes coding O-GlcNAc cycling enzymes in breast ductal carcinomas and clinicopathological parameters were analyzed. The results showed that poorly differentiated tumors (grade II and III) had significantly higher OGT expression than grade I tumors. Contrary, MGEA5 transcript levels were significantly lower in grade II and III in comparison with grade I tumors. The Spearman rank correlation showed the expressions of OGT and MGEA5 in breast cancer was negatively correlated (r = −0.430, P = 0.0002). Lymph node metastasis status was significantly associated with decreased MGEA5 mRNA expression. This result suggests that elevation in O-GlcNAc modification of proteins may be implicated in breast tumor progression and metastasis.
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影响因子：
21.3
作者：
Yang, Won Ho;Kim, Ji Eun;Cho, Jin Won
通讯作者：
Cho, Jin Won
影响因子：
3
作者：
Hanover, John A.;Krause, Michael W.;Love, Dona C.
通讯作者：
Love, Dona C.
影响因子：
13.8
作者：
Slawson, C.;Copeland, R. J.;Hart, G. W.
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DOI：
10.1016/j.biocel.2009.03.008
发表时间：
2009-11
影响因子：
4
作者：
Lazarus, Brooke D.;Love, Dona C.;Hanover, John A.
通讯作者：
Hanover, John A.
影响因子：
8
作者：
Caldwell, S. A.;Jackson, S. R.;Reginato, M. J.
通讯作者：
Reginato, M. J.