GABA estimation in the brains of children on the autism spectrum: measurement precision and regional cortical variation.
GABA estimation in the brains of children on the autism spectrum: measurement precision and regional cortical variation.
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DOI：
10.1016/j.neuroimage.2013.05.068
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发表时间：
2014-02-01
期刊：
影响因子：
5.7
通讯作者：
Roberts, T. P. L.
中科院分区：
文献类型：
--
作者：
Gaetz, W.;Bloy, L.;Wang, D. J.;Port, R. G.;Blaskey, L.;Levy, S. E.;Roberts, T. P. L.
1H-磁共振波谱（1H-MRS）和谱编辑方法，如MEGA-PRESS，使研究人员能够研究体内代谢物和神经递质浓度。在这里，我们解决利用1H-MRS的GABA浓度在ASD大脑中的调查，在三个位置，运动，视觉和听觉领域。初始重复性研究（5名受试者，5次重复测量，平均间隔约5天）表明参考代谢物选择对GABA定量无显著影响（p > 0.6）。GABA ~+/NAA、GABA ~+/Cr和GABA ~+/Glx的变异系数均在9- 11%之间。基于这些发现，我们调查了一组（n=17）自闭症谱系障碍（ASD）和（n = 17）典型发育儿童（TD）的运动，听觉和视觉感兴趣区域（ROI）的肌酸标准化GABA+比值（GABA+/Cr）。对灰质（GM）体积变化（已知随着发育而发生）的线性回归分析显示，随着运动年龄（F（1，30）=17.92; p<0.001）和视觉F（1，16）=14.41; p<0.005，但听觉感兴趣区（ROI）却没有显着减少（p=0.55）。GABA+/Cr随年龄变化的检查显示，仅运动ROI发生轻微显著变化（F（1，30）=4.11; p=0.054）。因此，使用年龄和GM体积作为协变量分别对每个ROI进行后续分析。对于TD与ASD儿童之间的视觉ROI，未观察到GABA+/Cr的组间差异。然而，运动和听觉ROI显示ASD中GABA+/Cr显著降低（运动p<0.05;听觉p<0.01）。运动ROI的GABA+/Cr平均不足约为11%，听觉ROI约为22%。我们的新发现支持ASD大脑中GABA+/Cr区域差异的模型，主要是在听觉和较小程度上的运动区域，但不是视觉区域。
1H-magnetic resonance spectroscopy (1H-MRS) and spectral editing methods, such as MEGA-PRESS, allow researchers to investigate metabolite and neurotransmitter concentrations in-vivo. Here we address the utilization of 1H-MRS for the investigation of GABA concentrations in the ASD brain, in three locations; motor, visual and auditory areas. An initial repeatability study (5 subjects, 5 repeated measures separated by ~ 5 days on average) indicated no significant effect of reference metabolite choice on GABA quantitation (p > 0.6). Coefficients of variation for GABA+/NAA, GABA+/Cr and GABA+/Glx were all of the order of 9–11%. Based on these findings, we investigated creatine-normalized GABA+ ratios (GABA+/Cr) in a group of (n=17) children with autism spectrum disorder (ASD) and (n=17) typically developing children (TD) for Motor, Auditory and Visual regions of interest (ROIs). Linear regression analysis of grey matter (GM) volume changes (known to occur with development) revealed a significant decrease of GM volume with Age for Motor (F(1,30)=17.92; p<0.001) and Visual F(1,16)=14.41; p<0.005 but not the Auditory ROI(p=0.55). Inspection of GABA+/Cr changes with Age revealed a marginally significant change for the Motor ROI only (F(1,30)=4.11; p=0.054). Subsequent analyses was thus conducted for each ROI separately using Age and GM volume as covariates. No group differences in GABA+/Cr were observed for the Visual ROI between TD vs. ASD children. However, the Motor and Auditory ROI showed significantly reduced GABA+/Cr in ASD (Motor p<0.05; Auditory p<0.01). The mean deficiency in GABA+/Cr from the Motor ROI was approximately 11% and Auditory ROI was approximately 22%. Our novel findings support the model of regional differences in GABA+/Cr in the ASD brain, primarily in Auditory and to a lesser extent Motor but not Visual areas.
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DOI：
10.1073/pnas.82.7.2148
发表时间：
1985-01-01
影响因子：
11.1
作者：
BOTTOMLEY, PA;EDELSTEIN, WA;ADAMS, WA
通讯作者：
ADAMS, WA
影响因子：
2.9
作者：
Geramita, Matthew;van der Veen, Jan Willem;Marenco, Stefano
通讯作者：
Marenco, Stefano
影响因子：
3.6
作者：
GROENENDAAL, F;VEENHOVEN, R;DEVRIES, LS
通讯作者：
DEVRIES, LS
影响因子：
10.6
作者：
Gandal, Michael J.;Edgar, J. Christopher;Ehrlichman, Richard S.;Mehta, Mili;Roberts, Timothy P. L.;Siegel, Steven J.
通讯作者：
Siegel, Steven J.
影响因子：
2.2
作者：
Collins, Ann L.;Ma, Deqiong;Whitehead, Patrice L.;Martin, Eden R.;Wright, Harry H.;Abramson, Ruth K.;Hussman, John P.;Haines, Jonathan L.;Cuccaro, Michael L.;Gilbert, John R.;Pericak-Vance, Margaret A.
通讯作者：
Pericak-Vance, Margaret A.