Sex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine Exposure.
Sex Differences in the Ventral Tegmental Area and Nucleus Accumbens Proteome at Baseline and Following Nicotine Exposure.
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DOI：
10.3389/fnmol.2021.657064
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发表时间：
2021
影响因子：
4.8
通讯作者：
Picciotto MR
中科院分区：
文献类型：
--
作者：
Lee AM;Mansuri MS;Wilson RS;Lam TT;Nairn AC;Picciotto MR
Sex differences in behaviors relevant to nicotine addiction have been observed in rodent models and human subjects. Behavioral, imaging, and epidemiological studies also suggest underlying sex differences in mesolimbic dopamine signaling pathways. In this study we evaluated the proteome in the ventral tegmental area (VTA) and nucleus accumbens (NAc) shell in male and female mice. Experimental groups included two mouse strains (C3H/HeJ and C57BL/6J) at baseline, a sub-chronic, rewarding regimen of nicotine in C3H/HeJ mice, and chronic nicotine administration and withdrawal in C57BL/6J mice. Isobaric labeling with a TMT 10-plex system, sample fractionation, and tandem mass spectrometry were used to quantify changes in protein abundance. In C3H/HeJ mice, similar numbers of proteins were differentially regulated between sexes at baseline compared with within each sex after sub-chronic nicotine administration. In C57BL/6J mice, there were significantly greater numbers of proteins differentially regulated between sexes at baseline compared with within each sex after chronic nicotine administration and withdrawal. Despite differences by sex, strain, and nicotine exposure parameters, glial fibrillary acidic protein (GFAP) and dopamine and cAMP-regulated phosphoprotein of 32 kDa (DARPP-32, Ppp1r1b) were repeatedly identified as significantly altered proteins, especially in the VTA. Further, network analyses showed sex- and nicotine-dependent regulation of a number of signaling pathways, including dopaminergic signaling. Sub-chronic nicotine exposure in female mice increased proteins related to dopaminergic signaling in the NAc shell but decreased them in the VTA, whereas the opposite pattern was observed in male mice. In contrast, dopaminergic signaling pathways were similarly upregulated in both male and female VTA after chronic nicotine and withdrawal. Overall, this study identifies significant sex differences in the proteome of the mesolimbic system, at baseline and after nicotine reward or withdrawal, which may help explain differential trajectories and susceptibility to nicotine addiction in males and females.
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影响因子：
3.3
作者：
Branson OE;Freitas MA
通讯作者：
Freitas MA
影响因子：
3.3
作者：
Becker, J. A. J.;Befort, K.;Blad, C.;Filliol, D.;Ghate, A.;Dembele, D.;Thibalilv, C.;Koch, M.;Muller, J.;Lardenois, A.;Poch, O.;Kieffer, B. L.
通讯作者：
Kieffer, B. L.
吸烟者和非吸烟者的纹状体多巴胺D2/D3受体可用性的性别差异。
DOI：
10.1017/s1461145711001957
发表时间：
2012-08
期刊：
The international journal of neuropsychopharmacology
影响因子：
--
作者：
Brown AK;Mandelkern MA;Farahi J;Robertson C;Ghahremani DG;Sumerel B;Moallem N;London ED
通讯作者：
London ED
影响因子：
--
作者：
Dani JA
通讯作者：
Dani JA
影响因子：
3
作者：
Bader, GD;Hogue, CW
通讯作者：
Hogue, CW