Transcriptome analysis identifies genes with enriched expression in the mouse central extended amygdala.
Transcriptome analysis identifies genes with enriched expression in the mouse central extended amygdala.
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DOI：
10.1016/j.neuroscience.2008.07.070
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发表时间：
2008-10-28
期刊：
影响因子：
3.3
通讯作者：
Kieffer, B. L.
中科院分区：
文献类型：
--
作者：
Becker, J. A. J.;Befort, K.;Blad, C.;Filliol, D.;Ghate, A.;Dembele, D.;Thibalilv, C.;Koch, M.;Muller, J.;Lardenois, A.;Poch, O.;Kieffer, B. L.
中央延伸杏仁核（EAc）是前脑边缘系统高度互连的结构的集合体，并形成包括终纹床核（BNST）、杏仁核中央核（CeA）和伏核壳（AcbSh）的细胞连续体。这个神经网络是大脑奖励和压力系统之间相互作用的关键部位，并且与药物滥用的几个方面有关。为了增加我们在分子水平上对EAc功能的理解，我们进行了全基因组筛选（Affyssin）以鉴定其表达在EAc中富集的基因。我们专注于EAc的不太为人所知的BNST-CeA区域，并鉴定了121个基因，与全脑相比，它们在EAc中的表达水平高出2倍以上。其中，43个基因从未被描述为在EAc中表达。我们绘制了这些基因在整个大脑中，使用非放射性原位杂交，并确定了8个基因具有独特的和不同的喙尾表达模式沿着AcbSh，BNST和CeA。在大脑和外周器官组织中进行的Q-PCR分析表明，除了一个基因（Spata 13）之外，所有这些基因主要在大脑中表达。这些基因编码信号蛋白（Adora 2，GPR 88，Arpp 21和Rem 2），转录因子（Limh 6）或功能未知的蛋白质（Rik 130，Spata 13和Wfs 1）。富集表达基因的鉴定扩展了我们在分子水平上对EAc的认识，并为EAc内的遗传操作提供了有用的信息。
The central Extended Amygdala (EAc) is an ensemble of highly interconnected limbic structures of the anterior brain, and forms a cellular continuum including the Bed Nucleus of the Stria Terminalis (BNST), the central nucleus of the Amygdala (CeA) and the Nucleus Accumbens shell (AcbSh). This neural network is a key site for interactions between brain reward and stress systems, and has been implicated in several aspects of drug abuse. In order to increase our understanding of EAc function at the molecular level, we undertook a genome-wide screen (Affymetrix) to identify genes whose expression is enriched in the EAc. We focused on the less-well known BNST-CeA areas of the EAc, and identified 121 genes that exhibit more than 2-fold higher expression level in the EAc compared to whole brain. Among these, forty-three genes have never been described to be expressed in the EAc. We mapped these genes throughout the brain, using non-radioactive in situ hybridization, and identified eight genes with a unique and distinct rostro-caudal expression pattern along AcbSh, BNST and CeA. Q-PCR analysis performed in brain and peripheral organ tissues indicated that, with the exception of one (Spata13), all these genes are predominantly expressed in brain. These genes encode signaling proteins (Adora2, GPR88, Arpp21 and Rem2), a transcription factor (Limh6) or proteins of unknown function (Rik130, Spata13 and Wfs1). The identification of genes with enriched expression expands our knowledge of EAc at a molecular level, and provides useful information to towards genetic manipulations within the EAc.
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