Intraoperative detection of IDH-mutant glioma using fluorescence lifetime imaging.
Intraoperative detection of IDH-mutant glioma using fluorescence lifetime imaging.
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DOI：
10.1002/jbio.202200291
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发表时间：
2023-04
影响因子：
2.8
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Noble Anbunesan, Silvia;Alfonso-Garcia, Alba;Zhou, Xiangnan;Bec, Julien;Lee, Han Sung;Jin, Lee-Way;Bloch, Orin;Marcu, Laura
在手术过程中而不是几天后识别异柠檬酸脱氢酶（IDH）突变和胶质瘤亚型可以帮助修改肿瘤切除策略，以获得更好的生存结果。我们报告了IDH突变型胶质瘤（N = 12例患者）的术中识别与临床兼容的荧光寿命成像（FLIm）设备（激发：355 nm;发射光谱带：390/40 nm，470/28 nm，542/50 nm）。分析荧光衍生的参数以研究IDH突变肿瘤和周围脑组织之间的光学对比度。IDH突变型少突胶质瘤的寿命（3.3 ± 0.1 ns）短于IDH突变型星形细胞瘤（4.1 ± 0.1 ns）。两种IDH突变型胶质瘤亚型的寿命均短于白色物质（4.6 ± 0.4 ns），但与皮质的寿命相当。IDH突变型少突胶质细胞瘤的生存期也随着恶性程度的增加而增加（2级：2.96 ± 0.08 ns，3级：3.4 ± 0.3 ns），但在IDH突变型星形细胞瘤中不增加。目前的结果支持FLIM作为鉴定IDH突变型胶质瘤组织的手术佐剂的可行性。
Identifying isocitrate dehydrogenase (IDH)-mutation and glioma subtype during surgery instead of days later can aid in modifying tumor resection strategies for better survival outcomes. We report intraoperative identification of IDH-mutant glioma (N = 12 patients) with a clinically compatible fluorescence lifetime imaging (FLIm) device (excitation: 355 nm; emission spectral bands: 390/40 nm, 470/28 nm, 542/50 nm). The fluorescence-derived parameters were analyzed to study the optical contrast between IDH-mutant tumors and surrounding brain tissue. IDH-mutant oligodendrogliomas exhibited shorter lifetimes (3.3 ± 0.1 ns) than IDH-mutant astrocytomas (4.1 ± 0.1 ns). Both IDH-mutant glioma subtypes had shorter lifetimes than white matter (4.6 ± 0.4 ns) but had comparable lifetimes to cortex. Lifetimes also increased with malignancy grade within IDH-mutant oligodendrogliomas (grade 2: 2.96 ± 0.08 ns, grade 3: 3.4 ± 0.3 ns) but not within IDH-mutant astrocytomas. The current results support the feasibility of FLIm as a surgical adjuvant for identifying IDH-mutant glioma tissue.
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影响因子：
13.8
作者：
Liberti MV;Locasale JW
通讯作者：
Locasale JW
影响因子：
1.4
作者：
Abdullah, Ahmed;El Shitany, Hisham;El Refaee, Ehab
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影响因子：
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Choi, Jinhyun;Kim, Se Hoon;Suh, Chang-Ok
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影响因子：
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作者：
Alfonso-Garcia A;Bec J;Weyers B;Marsden M;Zhou X;Li C;Marcu L
通讯作者：
Marcu L
影响因子：
3.4
作者：
Ma, Dinglong;Bec, Julien;Marcu, Laura
通讯作者：
Marcu, Laura