Functional slit lamp biomicroscopy for imaging bulbar conjunctival microvasculature in contact lens wearers.
Functional slit lamp biomicroscopy for imaging bulbar conjunctival microvasculature in contact lens wearers.
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DOI：
10.1016/j.mvr.2014.01.005
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发表时间：
2014-03
影响因子：
3.1
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Jiang H;Zhong J;DeBuc DC;Tao A;Xu Z;Lam BL;Liu C;Wang J
开发、测试和验证功能裂隙灯生物显微镜(FSLB)，以生成无创球结膜微血管灌注图(NMPM)，并评估形态测量和血流动力学。FSLB是从传统的裂隙灯显微镜改装而来的，通过连接数码相机对球结膜成像，创建nMPM并测量静脉血流动力学。在颞球结膜上获得大视野的高清晰度图像，用于建立nMPM。利用相机继承的高速设置和电影裁剪功能，血流动力学成像获得了60帧/秒的高成像速率和~210倍的高倍率。开发定制软件分割球结膜nMPM，进行进一步的分形分析，并定量测量血管直径、血流速度和流速。6名受试者在佩戴隐形眼镜6小时前和之后进行了成像。用单分形和多重分维分析来量化nMPM的分形性。球结膜血管直径由术前的18.8±2.7μm增加到戴镜6h后的19.6±2.4μm(P=0.020)。血流速度由0.60±0.12 mm/S增至0.88±0.21 mm/S(P=0.001)。血流量由129.8±59.9pl/S增至207.2±81.3pl/S(P=0.001)。球结膜nMPMS显示球结膜微血管网络的复杂细节。基线时，单分形分析分维为1.63±0.05，多重分维分析为1.71±0.03。戴镜6小时后，分维显著增加(P<；0.05)。利用FSLB可以方便、可靠地测量人球结膜微血管网的分形性、形态计量学和血流动力学。隐形眼镜配戴过程中的分维、形态计量学和血流动力学的变化可能预示着眼部微血管对配戴隐形眼镜的反应。
To develop, test and validate functional slit lamp biomicroscopy (FSLB) for generating non-invasive bulbar conjunctival microvascular perfusion maps (nMPMs) and assessing morphometry and hemodyanmics. FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the bulbar conjunctiva to create nMPMs and measure venular blood flow hemodyanmics. High definition images with a large field of view were obtained on the temporal bulbar conjunctiva for creating nMPMs. A high imaging rate of 60 frame per second and a ~210× high magnification were achieved using the camera inherited high speed setting and movie crop function, for imaging hemodyanmics. Custom software was developed to segment bulbar conjunctival nMPMs for further fractal analysis and quantitatively measure blood vessel diameter, blood flow velocity and flow rate. Six human subjects were imaged before and after 6 hours of wearing contact lenses. Monofractal and multifractal analyses were performed to quantify fractality of the nMPMs. The mean bulbar conjunctival vessel diameter was 18.8 ± 2.7 μm at baseline and increased to 19.6 ± 2.4 μm after 6 hours of lens wear (P = 0.020). The blood flow velocity was increased from 0.60 ± 0.12 mm/s to 0.88 ± 0.21 mm/s (P = 0.001). The blood flow rate was also increased from 129.8 ± 59.9 pl/s to 207.2 ± 81.3 pl/s (P = 0.001). Bulbar conjunctival nMPMs showed the intricate details of the bulbar conjunctival microvascular network. At baseline, fractal dimension was 1.63 ± 0.05 and 1.71 ± 0.03 analyzed by monofractal and multifractal analysis, respectively. Significant increases in fractal dimensions were found after 6 hours of lens wear (P < 0.05). Microvascular network’s fractality, morphometry and hemodyanmics of the human bulbar conjunctiva can be measured easily and reliably using FSLB. The alternations of the fractal dimensions, morphometry and hemodyanmics during contact lens wear may indicate ocular microvascular responses to contact lens wear.
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影响因子：
16.2
作者：
Cheung, Ning;Donaghue, Kim C.;Liew, Gerald;Rogers, Sophie L.;Wang, Jie Jin;Lim, Shueh-Wen;Jenkins, Alicia J.;Hsu, Wynne;Lee, Mong Li;Wong, Tien Y.
通讯作者：
Wong, Tien Y.
影响因子：
11.2
作者：
Cheung, Ning;Liew, Gerald;Wong, Tien Y.
通讯作者：
Wong, Tien Y.
影响因子：
9.9
作者：
Doubal, F. N.;MacGillivray, T. J.;Wardlaw, J. M.
通讯作者：
Wardlaw, J. M.
DOI：
10.1186/2040-7378-5-4
发表时间：
2013-03-14
期刊：
Experimental & translational stroke medicine
影响因子：
--
作者：
Schreiber S;Bueche CZ;Garz C;Braun H
通讯作者：
Braun H
影响因子：
5.7
作者：
Deneux, Thomas;Takerkart, Sylvain;Vanzetta, Ivo
通讯作者：
Vanzetta, Ivo