Genome-wide digital transcript analysis of putative fruitlet abscission related genes regulated by ethephon in litchi.
Genome-wide digital transcript analysis of putative fruitlet abscission related genes regulated by ethephon in litchi.
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荔枝中乙烯利调控的假定果实脱落相关基因的全基因组数字转录分析。
DOI：
10.3389/fpls.2015.00502
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发表时间：
2015
影响因子：
5.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Li C;Wang Y;Ying P;Ma W;Li J
荔枝（Litchi chinensis Sonn.）导致严重的产量损失。细胞分离发生在果实成熟区（FAZ），并可由乙烯引发。然而，对FAZ中发生的分子事件的深入了解仍然是未知的。本研究利用全基因组数字转录本丰度（DTA）技术分析了乙烯利对荔枝果实脱落相关基因的调控。通过高通量测序从七个乙烯利处理和未处理的对照文库获得超过8100万个高质量读数。通过DTA图谱分析，结合Gene Ontology和KEGG途径富集分析，共获得2730个与乙烯利促进荔枝幼果脱落相关的候选基因。其中，有1867个早期反应基因的表达上调或下调，从0到1天后处理。受影响最大的基因包括那些与乙烯生物合成和信号，生长素运输和信号，转录因子（TF），蛋白质泛素化，活性氧反应，钙信号转导和细胞壁修饰。根据基因表达模式的相似性，将这些基因分为4类13个亚类。qRT-PCR显示了41个候选基因的表达模式，证明了DTA数据的准确性。乙烯利处理显著提高了幼果的脱落率和乙烯释放量。提出了控制乙烯利促进荔枝幼果脱落的可能分子事件。幼果中乙烯释放量的增加会抑制生长素的合成和极性运输，从而引发乙烯释放信号。据我们所知，这是第一次在全基因组水平上监测乙烯利诱导荔枝果实脱落过程中FAZ富集花梗中发生的基因表达谱。本研究将有助于更好地了解荔枝果实脱落的分子调控机制。
The high level of physiological fruitlet abscission in litchi (Litchi chinensis Sonn.) causes severe yield loss. Cell separation occurs at the fruit abscission zone (FAZ) and can be triggered by ethylene. However, a deep knowledge of the molecular events occurring in the FAZ is still unknown. Here, genome-wide digital transcript abundance (DTA) analysis of putative fruit abscission related genes regulated by ethephon in litchi were studied. More than 81 million high quality reads from seven ethephon treated and untreated control libraries were obtained by high-throughput sequencing. Through DTA profile analysis in combination with Gene Ontology and KEGG pathway enrichment analyses, a total of 2730 statistically significant candidate genes were involved in the ethephon-promoted litchi fruitlet abscission. Of these, there were 1867 early-responsive genes whose expressions were up- or down-regulated from 0 to 1 d after treatment. The most affected genes included those related to ethylene biosynthesis and signaling, auxin transport and signaling, transcription factors (TFs), protein ubiquitination, ROS response, calcium signal transduction, and cell wall modification. These genes could be clustered into four groups and 13 subgroups according to their similar expression patterns. qRT-PCR displayed the expression pattern of 41 selected candidate genes, which proved the accuracy of our DTA data. Ethephon treatment significantly increased fruit abscission and ethylene production of fruitlet. The possible molecular events to control the ethephon-promoted litchi fruitlet abscission were prompted out. The increased ethylene evolution in fruitlet would suppress the synthesis and polar transport of auxin and trigger abscission signaling. To the best of our knowledge, it is the first time to monitor the gene expression profile occurring in the FAZ-enriched pedicel during litchi fruit abscission induced by ethephon on the genome-wide level. This study will contribute to a better understanding for the molecular regulatory mechanism of fruit abscission in litchi.
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影响因子：
6.9
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
5.3
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
7.4
作者：
Kwak, JM;Nguyen, V;Schroeder, JI
通讯作者：
Schroeder, JI
影响因子：
4
作者：
Iglesias, DJ;Tadeo, FR;Talon, M
通讯作者：
Talon, M
影响因子：
7.4
作者：
BEYER, EM;QUEBEDEAUX, B
通讯作者：
QUEBEDEAUX, B