Living bacterial sacrificial porogens to engineer decellularized porous scaffolds.
Living bacterial sacrificial porogens to engineer decellularized porous scaffolds.
复制标题
DOI：
10.1371/journal.pone.0019344
复制
发表时间：
2011-04-28
期刊：
影响因子：
3.7
通讯作者：
Demirci U
中科院分区：
文献类型：
--
作者：
Xu F;Sridharan B;Durmus NG;Wang S;Yavuz AS;Gurkan UA;Demirci U
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques suffer from limited control over pore interconnectivity, density and size, which leads to inefficient nutrient and oxygen transport to cells embedded in the scaffolds. Here, we demonstrated an innovative approach to develop a new platform for tissue engineered constructs using live bacteria as sacrificial porogens. E.coli were patterned and cultured in an interconnected three-dimensional (3D) hydrogel network. The growing bacteria created interconnected micropores and microchannels. Then, the scafold was decellularized, and bacteria were eliminated from the scaffold through lysing and washing steps. This 3D porous network method combined with bioprinting has the potential to be broadly applicable and compatible with tissue specific applications allowing seeding of stem cells and other cell types.
登录
查看更多内容
DOI：
10.1073/pnas.0910426107
发表时间：
2010-05-25
影响因子：
11.1
作者：
Di Leonardo, R.;Angelani, L.;Di Fabrizio, E.
通讯作者：
Di Fabrizio, E.
影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
影响因子：
3.4
作者：
Delaney, Joseph T., Jr.;Liberski, Albert R.;Schubert, Ulrich S.
通讯作者：
Schubert, Ulrich S.
影响因子：
7.4
作者：
Heo, J;Thomas, KJ;Crooks, RM
通讯作者：
Crooks, RM
DOI：
10.1016/j.msec.2007.11.008
发表时间：
2008-12-01
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Hamasaki, Shinichi;Tachibana, Akira;Tanabe, Toshizumi
通讯作者：
Tanabe, Toshizumi