Ion channels in Drosophila.
Ion channels in Drosophila.
复制标题
果蝇中的离子通道。
DOI：
10.1146/annurev.ph.50.030188.002115
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发表时间：
1988
影响因子：
18.2
通讯作者：
L. Jan
中科院分区：
文献类型：
--
作者：
D. Papazian;T. Schwarz;B. Tempel;L. Timpe;L. Jan
在这篇综述中，我们探索了利用黑腹果蝇的经典遗传学和分子遗传学研究离子通道结构和功能的方法。有两种不同的方法可以用来分离果蝇中离子通道的基因。在一种方法中，经典的遗传技术用于产生可能具有缺陷通道的突变果蝇。通常，这些突变体最初被认为是有趣的，因为它们的行为异常。随后，他们进行了电生理学研究，以了解突触传递或离子电流是否受到突变的影响。一旦鉴定出生理学上感兴趣的基因并确定其染色体位置，就可以克隆该基因。这种方法可以导致通道基因及其编码的蛋白质的分子分析，即使通道之前没有被纯化。在第二种方法中，已在其他生物中克隆的通道基因被用于从果蝇中分离同源基因。在将这些基因定位到染色体染色体位置后，可以使用经典的遗传技术来获得缺失和突变。这些突变的表型可以揭示野生型基因产物在体内的功能。第二种方法有时被称为“反向遗传学”。“一旦克隆了通道基因并分离出突变体，就可以应用果蝇的分子遗传技术。例如，P元件介导的野生型基因向突变果蝇的转化（61，75）应恢复正常功能。此外，通过编码序列或潜在的突变体的位点特异性诱变在体外产生突变等位基因，
In this review we explore the ways in which the classical and molecular genetics of Drosophila melanogaster can be used to study the structure and function of ion channels. Two different approaches can be used to isolate the genes for ion channels in Drosophila. In one approach, classical genetic techniques are used to generate mutant flies that may have defective channels. Often, these mutants are initially identified as interesting because they behave abnormally. Sub sequently, they are studied electrophysiologically to see whether synaptic transmission or ionic currents have been affected by the mutation. Once a physiologically interesting gene has been identified and its chromosomal location has been determined, the gene can be cloned. This approach can lead to molecular analysis of channel genes and the proteins they encode, even if the channel has not previously been purified. In the second approach, channel genes that have been cloned in other organisms are used to isolate homologous genes from Drosophila. After mapping these genes to chromo somal locations, classical genetic techniques can be used to obtain deletions and mutations. The phenotype of these mutations can reveal the function of the wild-type gene product in vivo. This second approach is sometimes referred to as "reverse genetics. " Once a channel gene has been cloned and mutants have been isolated, the molecular genetic techniques of Drosophila can be applied. For example, P element-mediated transformation (61, 75) of the wild-type gene into mutant flies should restore normal function. Furthermore, mutant alleles, generated in vitro by site-specific mutagenesis of either coding sequences or potential
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DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Messner,DJ;Catterall,WA
通讯作者：
Catterall,WA
果蝇离子通道的遗传学和分子生物学。
影响因子：
13.9
作者：
Tanouye,MA;Kamb,CA;Iverson,LE;Salkoff,L
通讯作者：
Salkoff,L
影响因子：
56.9
作者：
WU, CF;GANETZKY, B;LIU, AX
通讯作者：
LIU, AX
DOI：
10.1073/pnas.81.1.155
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FINERMOORE, J;STROUD, RM
通讯作者：
STROUD, RM