The nuclear protein Sge1 of Fusarium oxysporum is required for parasitic growth.
The nuclear protein Sge1 of Fusarium oxysporum is required for parasitic growth.
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DOI：
10.1371/journal.ppat.1000637
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发表时间：
2009-10
期刊：
影响因子：
6.7
通讯作者：
Rep M
中科院分区：
文献类型：
--
作者：
Michielse CB;van Wijk R;Reijnen L;Manders EM;Boas S;Olivain C;Alabouvette C;Rep M
二形性或形态转换是几种病原真菌利用的，是组织入侵和/或在宿主中生存所必需的。我们已经在植物病原真菌尖孢镰刀菌f.sp.中鉴定了这种形态转换的主要调节因子的同源基因。番茄。这种非二形性真菌通过穿透植物根部并定植维管组织，导致番茄发生维管萎缩病。基因敲除和互补研究证实，这种假定的调节因子SGE1(六基因表达1)的基因对致病性是必不可少的。此外，利用荧光蛋白进行的显微分析表明，Sge1定位于细胞核中，对根的定植和穿透不是必需的，但对寄生生长是必需的。此外，Sge1是表达感染期间分泌的效应器编码基因所必需的。我们认为尖孢镰刀菌和其他非二态(植物)病原真菌寄生生长需要Sge1。植物病原真菌已经进化出多种感染寄主的方式，并可能对商业作物生产产生毁灭性的影响。剖析它们的感染策略和了解参与致病的分子途径一直是并将继续是深入研究的主题。获得的新见解可能有助于制定疾病控制战略。尖孢镰刀菌已成为根部侵染、致病真菌的典范。这种真菌侵袭世界各地的多种植物，田间唯一有效的疾病控制策略是轮作和使用抗病植物品种(如果有的话)。在过去的十年中，已经发现了许多在致病过程中发挥作用的基因，其中许多与一般菌株的适应性有关。只有少数几个基因被确定为致病所必需的，但不影响营养生长。本文描述了这种基因的特征。它的蛋白产物Sge1在真菌王国中是保守的，代表了一类新的转录调节因子，参与了二态真菌病原体的形态转换。在尖孢镰刀菌中，Sge1蛋白是寄生生长所必需的，并与寄生阶段特定基因的表达有关。我们认为Sge1的功能在植物病原真菌中是保守的。
Dimorphism or morphogenic conversion is exploited by several pathogenic fungi and is required for tissue invasion and/or survival in the host. We have identified a homolog of a master regulator of this morphological switch in the plant pathogenic fungus Fusarium oxysporum f. sp. lycopersici. This non-dimorphic fungus causes vascular wilt disease in tomato by penetrating the plant roots and colonizing the vascular tissue. Gene knock-out and complementation studies established that the gene for this putative regulator, SGE1 (SIX Gene Expression 1), is essential for pathogenicity. In addition, microscopic analysis using fluorescent proteins revealed that Sge1 is localized in the nucleus, is not required for root colonization and penetration, but is required for parasitic growth. Furthermore, Sge1 is required for expression of genes encoding effectors that are secreted during infection. We propose that Sge1 is required in F. oxysporum and other non-dimorphic (plant) pathogenic fungi for parasitic growth. Plant pathogenic fungi have evolved many ways to infect their hosts and can have devastating effects on commercial crop production. Dissecting their infection strategies and understanding the molecular pathways involved in pathogenesis have been and continue to be the subject of intensive research. New insights gained may help to develop disease controlling strategies. Fusarium oxysporum has become a model for root invading, pathogenic fungi. This fungus attacks a wide range of plant species worldwide and the only effective disease control strategies in the field are crop rotation and usage of resistant plant varieties, if at all available. In the last decade, many genes have been identified that play a role during pathogenesis, many of which are linked to general strain fitness. Only few genes have been identified that are required for pathogenesis but do not affect vegetative growth. This paper describes the characterization of such a gene. Its protein product, Sge1, is conserved in the fungal kingdom and represents a new class of transcriptional regulators involved in morphological switching in dimorphic fungal pathogens. In F. oxysporum, the Sge1 protein is required for parasitic growth and is associated with expression of parasitic phase-specific genes. We suggest that the function of Sge1 is conserved in (plant) pathogenic fungi.
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