Nuclear mechanical resilience but not stiffness is modulated by αII-spectrin.
Nuclear mechanical resilience but not stiffness is modulated by αII-spectrin.
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DOI：
10.1016/j.jbiomech.2016.10.034
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发表时间：
2016-12-08
影响因子：
2.4
通讯作者：
Dahl, Kris Noel
中科院分区：
文献类型：
--
作者：
Armiger, Travis J.;Spagnol, Stephen T.;Dahl, Kris Noel
Spectrins are multi-domain, elastic proteins that provide elasticity to the plasma membrane of erythrocytes and select nucleated cells. Spectrins have also been found in the nucleus of non-erythrocytes, but their function remains to be uncovered. It has been hypothesized that a spring-like spectrin network exists within the lamina nucleoskeleton, however, experiments testing a spectrin network’s mechanical impact on the nucleus are lacking. Here, we knock-down levels of nuclear αII-spectrin with the goal of disrupting this nucleoskeletal spectrin network. We mechanically test live cells with intranuclear particle tracking and compression assays to probe changes in nuclear mechanics with decreases in αII-spectrin. We show no changes in chromatin mechanics or in the stiffness of nuclei under compression. However, we do observe a reduction in the ability of nuclei with decreased αII-spectrin to recover after compression. These results establish spectrin as a nucleoskeletal component that specifically contributes to elastic recovery after compression.
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影响因子：
3.3
作者：
Shimi T;Kittisopikul M;Tran J;Goldman AE;Adam SA;Zheng Y;Jaqaman K;Goldman RD
通讯作者：
Goldman RD
DOI：
10.1083/jcb.200801105
发表时间：
2008-08-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yang G;Cameron LA;Maddox PS;Salmon ED;Danuser G
通讯作者：
Danuser G
影响因子：
4.8
作者：
Metral, Sylvain;Machnicka, Beata;Lecomte, Marie-Christine
通讯作者：
Lecomte, Marie-Christine
影响因子：
3.9
作者：
Sridharan, Deepa M.;McMahon, Laura W.;Lambert, Muriel W.
通讯作者：
Lambert, Muriel W.
影响因子：
8
作者：
Machnicka, B.;Grochowalska, R.;Boguslawska, D. M.;Sikorski, A. F.;Lecomte, M. C.
通讯作者：
Lecomte, M. C.