Isolation of a cell surface receptor protein for laminin from murine fibrosarcoma cells.
Isolation of a cell surface receptor protein for laminin from murine fibrosarcoma cells.
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DOI：
10.1083/jcb.96.5.1475
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发表时间：
1983-05
期刊：
影响因子：
--
通讯作者：
Wicha MS
中科院分区：
文献类型：
--
作者：
Malinoff HL;Wicha MS
我们使用亲和层析从小鼠纤维肉瘤细胞中分离出一种特异性层粘连蛋白结合蛋白。这些细胞以高亲和力（对于层粘连蛋白，Kd = 2 × 10（-9）M）将外源层粘连蛋白结合到其表面，每个细胞具有约5 × 10（4）个位点。[35 S]甲硫氨酸标记的细胞提取物的层粘连蛋白亲和层析产生两种不同的蛋白质。根据其在SDS凝胶上的迁移模式和细菌胶原酶敏感性，将一种鉴定为IV型（基底膜）胶原。另一种蛋白质在还原SDS凝胶上以单一条带或紧密间隔的双峰形式迁移，分子量降低至69，000。用硝酸纤维素滤纸片分析，我们发现后者蛋白特异性结合125 I-层粘连蛋白，其亲和力与完整纤维肉瘤细胞相同（层粘连蛋白的Kd = 2 × 10（-9）M）。通过碘标记完整的细胞，我们证明了这种层粘连蛋白结合蛋白是在细胞表面。我们的结论是，这种蛋白质与减少分子量为69,000是一个亚基或组件的一个较大的细胞表面受体蛋白层粘连蛋白在这个纤维肉瘤模型。这种层粘连蛋白受体可以介导细胞与其细胞外基质的相互作用。
We used affinity chromatography to isolate a specific laminin-binding protein from murine fibrosarcoma cells. These cells bind exogenous laminin to their surface with high affinity (Kd = 2 X 10(-9)M for laminin) with approximately 5 X 10(4) sites per cell. Laminin affinity chromatography of [35S]methionine-labeled cell extracts produced two distinct proteins. One was identified as Type IV (basement membrane) collagen based on its migration pattern on SDS gels and bacterial collagenase sensitivity. The other protein, which migrates as a single band or closely spaced doublet on reduced SDS gels, has a reduced molecular weight of 69,000. Using a nitrocellulose filter disk assay, we found that the latter protein specifically bound 125I-laminin with the same high affinity (Kd = 2 X 10(-9)M for laminin) as did intact fibrosarcoma cells. By iodinating intact cells, we demonstrated that this laminin-binding protein is on the cell surface. We conclude that this protein with reduced molecular weight of 69,000 is a subunit or component of a larger cell surface receptor protein for laminin in this fibrosarcoma model. This laminin receptor may mediate the interaction of the cell with its extracellular matrix.
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影响因子：
64.8
作者：
VLODAVSKY, I;GOSPODAROWICZ, D
通讯作者：
GOSPODAROWICZ, D
影响因子：
64.5
作者：
TERRANOVA, VP;ROHRBACH, DH;MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
64.8
作者：
LAEMMLI, UK
通讯作者：
LAEMMLI, UK
影响因子：
2.9
作者：
PETERKOFSKY, B;DIEGELMANN, R
通讯作者：
DIEGELMANN, R
DOI：
10.1073/pnas.80.2.444
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
TERRANOVA, VP;RAO, CN;LIOTTA, LA
通讯作者：
LIOTTA, LA