Signalling of Arabidopsis thaliana response to Pieris brassicae eggs shares similarities with PAMP-triggered immunity.
Signalling of Arabidopsis thaliana response to Pieris brassicae eggs shares similarities with PAMP-triggered immunity.
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DOI：
10.1093/jxb/ers362
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发表时间：
2013-01
影响因子：
6.9
通讯作者：
Reymond P
中科院分区：
文献类型：
--
作者：
Gouhier-Darimont C;Schmiesing A;Bonnet C;Lassueur S;Reymond P
昆虫卵沉积激活植物防御，但很少有人知道控制这种反应的信号事件。在拟南芥中，菜粉蝶产卵触发水杨酸（SA）的积累，诱导防御基因的表达。这类似于病原体相关分子模式（PAMP）的识别，其参与PAMP触发的免疫（PTI）。在这里，参与已知的信号组件的PTI在产卵进行了研究。用芸苔疫霉卵提取物处理引起早期PAMP响应基因的快速诱导。此外，防御基因PR-1的表达需要EDS 1、SID 2和部分NPR 1，从而暗示SA途径下游的卵识别。PR-1的表达是由鸡蛋提取物的非极性组分和通过EDS 1和NUDT 7的拮抗作用调节的氧化爆发触发的，但不依赖于NADPH氧化酶RBOHD和RBOHF。通过对卵源性激发子受体的研究，发现了一种受体样激酶突变体lecRK-I.8，其对卵提取物处理后PR-1的诱导作用显著降低。这些结果证明了SA途径在响应卵源激发子中的重要性，并揭示了拟南芥中昆虫卵和PTI检测之间有趣的相似性。
Insect egg deposition activates plant defence, but very little is known about signalling events that control this response. In Arabidopsis thaliana, oviposition by Pieris brassicae triggers salicylic acid (SA) accumulation and induces the expression of defence genes. This is similar to the recognition of pathogen-associated molecular patterns (PAMPs), which are involved in PAMP-triggered immunity (PTI). Here, the involvement of known signalling components of PTI in response to oviposition was studied. Treatment with P. brassicae egg extract caused a rapid induction of early PAMP-responsive genes. In addition, expression of the defence gene PR-1 required EDS1, SID2, and, partially, NPR1, thus implicating the SA pathway downstream of egg recognition. PR-1 expression was triggered by a non-polar fraction of egg extract and by an oxidative burst modulated through the antagonistic action of EDS1 and NUDT7, but which did not depend on the NADPH oxidases RBOHD and RBOHF. Searching for receptors of egg-derived elicitors, a receptor-like kinase mutant, lecRK-I.8, was identified which shows a much reduced induction of PR-1 in response to egg extract treatment. These results demonstrate the importance of the SA pathway in response to egg-derived elicitor(s) and unravel intriguing similarities between the detection of insect eggs and PTI in Arabidopsis.
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影响因子：
2.9
作者：
Bruessow, Friederike;Reymond, Philippe
通讯作者：
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