Bimodal signatures of germline methylation are linked with gene expression plasticity in the coral Acropora millepora.
Bimodal signatures of germline methylation are linked with gene expression plasticity in the coral Acropora millepora.
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DOI：
10.1186/1471-2164-15-1109
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发表时间：
2014-12-15
期刊：
影响因子：
4.4
通讯作者：
Matz MV
中科院分区：
文献类型：
--
作者：
Dixon GB;Bay LK;Matz MV
在无脊椎动物中，属于动态调节功能类别的基因似乎比“管家”基因的甲基化程度低，这表明 DNA 甲基化可能会调节基因表达的可塑性。然而，迄今为止，缺乏在不同自然栖息地支持这一假设的实验证据。基因表达谱是由 30 对基因相同的鹿角珊瑚片段生成的，这些片段在不同的自然栖息地之间相互移植 3 个月。在标准化 CpG 含量的背景下分析基因表达，CpG 含量是历史种系 DNA 甲基化的公认特征。与具有强甲基化特征的基因相比，具有弱甲基化特征的基因更有可能表现出基于移植环境和起源群体的差异表达。此外，对于具有弱甲基化特征的基因，由于环境和群体而导致的表达差异的程度更大。我们的结果支持差异种系甲基化与跨环境和人群的基因表达灵活性之间的联系。对珊瑚等系统发育基础无脊椎动物的研究将进一步阐明后生动物基因体甲基化的基本功能。本文的在线版本 (doi:10.1186/1471-2164-15-1109) 包含补充材料，可供授权用户使用。
In invertebrates, genes belonging to dynamically regulated functional categories appear to be less methylated than “housekeeping” genes, suggesting that DNA methylation may modulate gene expression plasticity. To date, however, experimental evidence to support this hypothesis across different natural habitats has been lacking. Gene expression profiles were generated from 30 pairs of genetically identical fragments of coral Acropora millepora reciprocally transplanted between distinct natural habitats for 3 months. Gene expression was analyzed in the context of normalized CpG content, a well-established signature of historical germline DNA methylation. Genes with weak methylation signatures were more likely to demonstrate differential expression based on both transplant environment and population of origin than genes with strong methylation signatures. Moreover, the magnitude of expression differences due to environment and population were greater for genes with weak methylation signatures. Our results support a connection between differential germline methylation and gene expression flexibility across environments and populations. Studies of phylogenetically basal invertebrates such as corals will further elucidate the fundamental functional aspects of gene body methylation in Metazoa. The online version of this article (doi:10.1186/1471-2164-15-1109) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Foden WB;Butchart SH;Stuart SN;Vié JC;Akçakaya HR;Angulo A;DeVantier LM;Gutsche A;Turak E;Cao L;Donner SD;Katariya V;Bernard R;Holland RA;Hughes AF;O'Hanlon SE;Garnett ST;Sekercioğlu CH;Mace GM
通讯作者：
Mace GM
蜜蜂转录组的表观遗传调控：揭示甲基化基因的本质。
影响因子：
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作者：
Fisher, RA
通讯作者：
Fisher, RA
影响因子：
5.2
作者：
Chevin, Luis-Miguel;Collins, Sinead;Lefevre, Francois
通讯作者：
Lefevre, Francois