Smartphone-based rapid quantitative detection of luteinizing hormone using gold immunochromatographic strip
Smartphone-based rapid quantitative detection of luteinizing hormone using gold immunochromatographic strip
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DOI：
10.1016/j.snb.2017.12.161
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Cai Xinxia
中科院分区：
文献类型：
--
作者：
Liu Juntao;Fan Yan;Kong Zhuang;Wang Yang;Luo Jinping;Xu Shengwei;Jin Hongyan;Cai Xinxia
黄体生成素(LH)水平的测定在辅助生殖、卵巢疾病的诊断和临床疗效评价等方面具有重要意义。本研究的目的是开发一种基于智能手机的定量方法，使用金免疫层析条(GICS)来测定促黄体生成素水平。在该方法中，使用智能手机获取GICS的图像，并使用Canny算子和模糊c-均值聚类算法提取GICS的测试线和控制线。为了减少环境干扰，提高GICS图像的检测一致性，采用了亮度补偿方法。实验结果表明，该方法有效地降低了测量的相对标准偏差，在1H1.0-83.3mIU/mL范围内具有良好的线性关系(r=0.996)，并且在不同类型的智能手机和GICSS上具有高度的重现性。将该方法进一步用于临床血清标本(n=31)的检测，与常规大型生化仪器的相关系数达到0.974，并跟踪志愿者排卵期间的尿促黄体生成素水平，在15分钟内获得定量结果。该方法高效、灵敏，可用于黄体生成素的快速定量检测。
Measurement of the luteinizing hormone (LH) level is very important in assisted reproduction, the diagnosis of ovarian diseases, and evaluation of clinical effects. The objective of the present study was the development of a smartphone-based quantitative method for determining the LH level using a gold immuno-chromatographic strip(GICS). In the developed method, a smartphone is used to obtain the image of the GICS and Canny operator and fuzzy c-means clustering algorithms are used to extract the test and control lines of the GICS. A luminance compensation method is used to reduce environmental interference and increase the detection consistency of the GICS image. Experimental results revealed that the proposed method effectively reduced the relative standard deviation (RSD) of the measurements, afforded high linearity within LH levels of 1.0–83.3 mIU/mL (r = 0.996), and was highly reproducible using different types of smartphones and GICSs. The proposed method was further used to detect clinical serum samples (n = 31), with the achievement of a corresponding correlation coefficient of 0.974 against conventional large biochemical instrument; and to track the urine LH level of a volunteer during ovulation, with quantitative results obtained within 15 min. The method was found to be highly efficient and sensitive and is thus potentially applicable to rapid quantitative LH testing.
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影响因子：
2
作者：
Wu CC;Lin HY;Wang CP;Lu LF;Yu TH;Hung WC;Houng JY;Chung FM;Lee YJ;Hu JJ
通讯作者：
Hu JJ
DOI：
10.1109/icma.2008.4798830
发表时间：
2008-08
期刊：
2008 IEEE International Conference on Mechatronics and Automation
影响因子：
--
作者：
Ekkalak Sumonphan;S. Auephanwiriyakul;N. Theera-Umpon
通讯作者：
Ekkalak Sumonphan;S. Auephanwiriyakul;N. Theera-Umpon
影响因子：
6.7
作者：
Ferraretti, AP;Gianaroli, L;Montanaro, N
通讯作者：
Montanaro, N
影响因子：
6.7
作者：
Ruvolo, Giovanni;Bosco, Liana;Roccheri, Maria C.
通讯作者：
Roccheri, Maria C.
影响因子：
4.3
作者：
M. Ujihara;K. Yamamoto;K. Nomura;S. Toyoshima;H. Demura;Y. Nakamura;K. Ohmura;T. Osawa
通讯作者：
M. Ujihara;K. Yamamoto;K. Nomura;S. Toyoshima;H. Demura;Y. Nakamura;K. Ohmura;T. Osawa