Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study.
Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study.
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DOI：
10.1016/j.ocarto.2022.100317
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发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Loeser, Richard F.
中科院分区：
文献类型：
--
作者：
Arbeeva, Liubov;Azcarate-Peril, M. Andrea;Cui, Yang;Nelson, Amanda E.;Loeser, Richard F.
研究伴有和不伴有骨关节炎（OA）的肥胖者血浆微生物组的差异及其与血清脂多糖（LPS）的关系。70名体重指数(BMI)≥30kg/m2，年龄≥55岁，有（病例）或没有（对照组）手膝OA的参与者的血液样本进行血清LPS和血浆微生物组组成分析。将dirichlet -多项式递归划分模型（DM-RPart）应用于微生物组组成数据，以验证LPS水平区分血浆微生物组的假设，考虑到BMI和年龄。在属水平上，病例和对照组之间α多样性或组间组成差异无显著性差异（p = 0.11）。β-多样性与血清LPS水平显著相关（p = 0.01）。DM-RPart得到的最优树有3个分区：1)基于年龄（在69岁时划分）；2)年龄大于69岁的按BMI划分；3) BMI <39 kg/m2组按脂多糖水平（65 EU/ml）分组。结果分为4组（节点2和节点5-7）。节点2的参与者较年轻，大多数没有或轻度OA。5号淋巴结和6号淋巴结的患者在年龄和BMI方面具有可比性，但6号淋巴结的LPS较高，OA更严重。节点7的个体年龄较大，BMI较高，OA最严重。我们的研究结果表明，在患有手膝关节炎的肥胖个体亚组中，血清LPS和血浆微生物组之间的关系可能反映了肠道通透性的差异。
To examine the plasma microbiome for differences between obese individuals with and without osteoarthritis (OA) and its association with serum lipopolysaccharide (LPS). Blood samples from 70 participants with body mass index (BMI) ≥ 30kg/m2 and age ≥55 years, with (cases) or without (controls) hand plus knee OA, were analyzed for serum LPS and composition of the plasma microbiome. The Dirichlet-multinominal recursive partitioning model (DM-RPart) was applied to microbiome compositional data to test the hypothesis that LPS levels distinguish plasma microbiome, accounting for BMI and age. No significant differences in alpha diversity, or compositional differences between groups at the genus level, were seen between cases and controls (p = 0.11). β-Diversity was significantly associated with serum LPS levels (p = 0.01). DM-RPart resulted in an optimal tree with 3 divisions: 1) based on age (split at 69 years); 2) those older than 69 were split based on BMI; 3) those with BMI <39 kg/m2 were split based on LPS level (at 65 EU/ml). This resulted in 4 groups (nodes 2, and 5–7). Participants in node 2 were younger and the majority had no or mild OA. Those in nodes 5 and 6 were comparable in age and BMI but node 6 had higher LPS and more severe OA. Individuals in node 7 were older, had higher BMI, and the most severe OA. Our results suggest a relationship between serum LPS and the plasma microbiome in a subgroup of obese individuals with hand plus knee OA that could reflect differences in intestinal permeability.
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DOI：
10.1177/1535370216681549
发表时间：
2017-02
期刊：
Experimental biology and medicine (Maywood, N.J.)
影响因子：
--
作者：
Pretorius E;Akeredolu OO;Soma P;Kell DB
通讯作者：
Kell DB
影响因子：
4.2
作者：
Allali I;Arnold JW;Roach J;Cadenas MB;Butz N;Hassan HM;Koci M;Ballou A;Mendoza M;Ali R;Azcarate-Peril MA
通讯作者：
Azcarate-Peril MA
影响因子：
1
作者：
Tommasi C;Equitani F;Masala M;Ballardini M;Favaro M;Meledandri M;Fontana C;Narciso P;Nicastri E
通讯作者：
Nicastri E
影响因子：
4.1
作者：
Ghosh, Siddhartha S.;Wang, Jing;Ghosh, Shobha
通讯作者：
Ghosh, Shobha
影响因子：
11.3
作者：
Potgieter M;Bester J;Kell DB;Pretorius E
通讯作者：
Pretorius E