Mycoplasma agassizii, an opportunistic pathogen of tortoises, shows very little genetic variation across the Mojave and Sonoran Deserts.
Mycoplasma agassizii, an opportunistic pathogen of tortoises, shows very little genetic variation across the Mojave and Sonoran Deserts.
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分枝杆菌Agassizii是一种机会性的乌龟病原体，在莫哈韦和索诺拉沙漠中几乎没有遗传变异。
DOI：
10.1371/journal.pone.0245895
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Alvarez-Ponce D
中科院分区：
文献类型：
--
作者：
Luzuriaga-Neira A;Sandmeier FC;Weitzman CL;Tracy CR;Bauschlicher SN;Tillett RL;Alvarez-Ponce D
阿加西支原体是莫哈韦沙漠龟（Gopherus agassizii）上呼吸道疾病的常见原因。到目前为止，这种细菌只有两种菌株被测序，并且对其遗传多样性模式知之甚少。了解这种病原体的遗传变异性对于实施对其受威胁的长寿宿主的保护计划至关重要。我们使用下一代测序技术来探索 2011 年和 2012 年从大部分健康的莫哈韦沙漠龟和索诺兰沙漠龟中收集的 86 个阿加西龟培养样本的基因组多样性。所有具有足够测序覆盖度的样本与阿加西龟菌株 PS6T（采集于内华达州拉斯维加斯谷）的相似性高于菌株 723（采集于佛罗里达州萨尼贝尔岛）。对所有八个测序覆盖率超过 2 倍的基因组进行了多次分析，以检测单核苷酸多态性 (SNP)。引人注目的是，尽管我们在菌株 PS6T 和 723 之间检测到了 1373 个 SNP，但我们没有在 PS6T 和我们的八个样本之间检测到任何 SNP。我们的全基因组分析表明，M. agassizii 菌株 PS6T 可能存在于健康的莫哈韦和索诺兰沙漠龟的广阔地理范围内。
Mycoplasma agassizii is a common cause of upper respiratory tract disease in Mojave desert tortoises (Gopherus agassizii). So far, only two strains of this bacterium have been sequenced, and very little is known about its patterns of genetic diversity. Understanding genetic variability of this pathogen is essential to implement conservation programs for their threatened, long-lived hosts. We used next generation sequencing to explore the genomic diversity of 86 cultured samples of M. agassizii collected from mostly healthy Mojave and Sonoran desert tortoises in 2011 and 2012. All samples with enough sequencing coverage exhibited a higher similarity to M. agassizii strain PS6T (collected in Las Vegas Valley, Nevada) than to strain 723 (collected in Sanibel Island, Florida). All eight genomes with a sequencing coverage over 2x were subjected to multiple analyses to detect single-nucleotide polymorphisms (SNPs). Strikingly, even though we detected 1373 SNPs between strains PS6T and 723, we did not detect any SNP between PS6T and our eight samples. Our whole genome analyses reveal that M. agassizii strain PS6T may be present across a wide geographic extent in healthy Mojave and Sonoran desert tortoises.
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DOI：
10.1093/bioinformatics/btp347
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Assefa S;Keane TM;Otto TD;Newbold C;Berriman M
通讯作者：
Berriman M
影响因子：
3
作者：
Korneliussen TS;Albrechtsen A;Nielsen R
通讯作者：
Nielsen R
影响因子：
3.3
作者：
Gonzales, Tina K.;Kulow, Megan;Doepfer, Doerte
通讯作者：
Doepfer, Doerte
影响因子：
3.3
作者：
Farmanfarmaei, Ghazaleh;Kamakoli, Mansour Kargarpour;Siadat, Seyed Davar
通讯作者：
Siadat, Seyed Davar
影响因子：
3.2
作者：
Becker, Claire A. M.;Thibault, Francois M.;Tardy, Florence
通讯作者：
Tardy, Florence