ABACAS: algorithm-based automatic contiguation of assembled sequences.

ABACAS: algorithm-based automatic contiguation of assembled sequences.
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DOI:
10.1093/bioinformatics/btp347
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发表时间:
2009-08-01
期刊:
Bioinformatics (Oxford, England)
影响因子:
--
通讯作者:
Berriman M
Berriman M
中科院分区:
其他
文献类型:
--
作者:
Assefa S;Keane TM;Otto TD;Newbold C;Berriman M

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总结:由于新的测序技术的可用性,我们现在越来越感兴趣的测序密切相关的菌株现有的成品基因组。最近,已经开发了许多从头和基于定位的组装器,以从新的测序技术读取产生高质量的基因组草案。新的工具是必要的,以采取重叠群从一个草案大会通过一个完全重叠的基因组序列。ABACAS旨在作为一种工具,用于快速重叠(比对,排序,定向),可视化和设计引物,以基于参考序列关闭鸟枪组装重叠群上的缺口。ABACAS的输入是一组重叠群,这些重叠群将与参考基因组进行比对,排序和定向,在ACT比较浏览器中可视化,并自动生成最佳引物序列。可用性和实现:ABACAS是用Perl实现的,可以从sa4@sanger.ac.uk免费下载。http://abacas.sourceforge.net
Summary: Due to the availability of new sequencing technologies, we are now increasingly interested in sequencing closely related strains of existing finished genomes. Recently a number of de novo and mapping-based assemblers have been developed to produce high quality draft genomes from new sequencing technology reads. New tools are necessary to take contigs from a draft assembly through to a fully contiguated genome sequence. ABACAS is intended as a tool to rapidly contiguate (align, order, orientate), visualize and design primers to close gaps on shotgun assembled contigs based on a reference sequence. The input to ABACAS is a set of contigs which will be aligned to the reference genome, ordered and orientated, visualized in the ACT comparative browser, and optimal primer sequences are automatically generated. Availability and Implementation: ABACAS is implemented in Perl and is freely available for download from http://abacas.sourceforge.net Contact: sa4@sanger.ac.uk
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