Regional Variation of Gap Junctional Connections in the Mammalian Inner Retina.
Regional Variation of Gap Junctional Connections in the Mammalian Inner Retina.
复制标题
哺乳动物内视网膜缝隙连接的区域性变化。
DOI：
10.3390/cells10092396
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发表时间：
2021-09-12
期刊：
影响因子：
6
通讯作者：
Telkes I
中科院分区：
文献类型：
--
作者：
Fusz K;Kovács-Öller T;Kóbor P;Szabó-Meleg E;Völgyi B;Buzás P;Telkes I
许多物种的视网膜显示出区域特化，这在处理来自视野不同部分的视觉输入的差异中是明显的。区域化被认为反映了对自然视觉环境、光学限制和物种生活方式的适应。然而，人们对突触回路的区域差异知之甚少。在这里，我们感兴趣的拓扑分布连接蛋白36（Cx 36），在视网膜电突触的主要组成部分。我们比较了小鼠、大鼠和猫的视网膜，以在分析中包括具有不同区域专业化模式的物种。首先，我们使用Prox 1免疫反应性无长突细胞的密度作为任何区域专业化的标志，细胞密度越高，意味着更多的中心区域。双标记实验表明，Prox 1在所有三个物种的AII无长突细胞中表达。有趣的是，大的Cx 36斑块附着在约8-10%的Prox 1阳性无长突细胞胞体上，这表明成对或小簇细胞体的强电耦合。当分析Cx 36免疫反应性斑块体积密度的区域变化时，我们发现所有三个物种的ON亚板中表达Prox 1的无长突细胞的密度密切相关，但在OFF亚板中则不然。结果表明，视网膜的ON和OFF通路的电突触的相对贡献的变化与视网膜的位置，这可能有助于整个视野的功能ON/OFF不对称。
The retinas of many species show regional specialisations that are evident in the differences in the processing of visual input from different parts of the visual field. Regional specialisation is thought to reflect an adaptation to the natural visual environment, optical constraints, and lifestyle of the species. Yet, little is known about regional differences in synaptic circuitry. Here, we were interested in the topographical distribution of connexin-36 (Cx36), the major constituent of electrical synapses in the retina. We compared the retinas of mice, rats, and cats to include species with different patterns of regional specialisations in the analysis. First, we used the density of Prox1-immunoreactive amacrine cells as a marker of any regional specialisation, with higher cell density signifying more central regions. Double-labelling experiments showed that Prox1 is expressed in AII amacrine cells in all three species. Interestingly, large Cx36 plaques were attached to about 8–10% of Prox1-positive amacrine cell somata, suggesting the strong electrical coupling of pairs or small clusters of cell bodies. When analysing the regional changes in the volumetric density of Cx36-immunoreactive plaques, we found a tight correlation with the density of Prox1-expressing amacrine cells in the ON, but not in the OFF sublamina in all three species. The results suggest that the relative contribution of electrical synapses to the ON- and OFF-pathways of the retina changes with retinal location, which may contribute to functional ON/OFF asymmetries across the visual field.
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DOI：
10.1523/jneurosci.6216-11.2012
发表时间：
2012-03-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Curti S;Hoge G;Nagy JI;Pereda AE
通讯作者：
Pereda AE
影响因子：
5.3
作者：
Escobar, Maria-Jose;Reyes, Cesar;Palacios, Adrian G.
通讯作者：
Palacios, Adrian G.
影响因子：
4
作者：
Fournel R;Hartveit E;Veruki ML
通讯作者：
Veruki ML
影响因子：
16.2
作者：
Brivanlou, IH;Warland, DK;Meister, M
通讯作者：
Meister, M
影响因子：
16.2
作者：
Deans, MR;Volgyi, B;Paul, DL
通讯作者：
Paul, DL