Stereotypic Immune System Development in Newborn Children.
Stereotypic Immune System Development in Newborn Children.
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DOI：
10.1016/j.cell.2018.06.045
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发表时间：
2018-08-23
期刊：
影响因子：
64.5
通讯作者：
Brodin P
中科院分区：
文献类型：
--
作者：
Olin A;Henckel E;Chen Y;Lakshmikanth T;Pou C;Mikes J;Gustafsson A;Bernhardsson AK;Zhang C;Bohlin K;Brodin P
流行病学数据表明，生命早期的暴露是日后免疫介导疾病的关键决定因素。幼儿也特别容易受到感染，因此需要对生命早期的免疫系统发育进行更多的分析。这种分析大多是在小鼠模型或人类脐带血样本中进行的，但这些分析不能解释影响人类新生儿出生后的复杂环境暴露。在这里，我们对100名新生儿进行了纵向分析，在他们生命的前3个月内采样多达4次。从100 μL的血液中，我们通过质谱仪分析了58个免疫细胞群的发育，通过免疫测定分析了267个血浆蛋白，发现了脐带血测量无法预测的剧烈变化，但遵循了刻板模式。早产儿和足月儿在出生时有所不同，但却趋同于一个共同的轨迹，似乎是由微生物相互作用驱动的，并受到早期肠道细菌生态失调的阻碍。脐带血不能代表出生后的免疫力早产儿和足月儿在出生时不同，但此后迅速收敛免疫系统的发展遵循一个刻板的模式在生命早期动态参数意味着微生物的相互作用在早期免疫发展的纵向分析血液免疫细胞从100个新生儿提供了一个系统的观点对人类新生儿免疫系统的个体发育。
Epidemiological data suggest that early life exposures are key determinants of immune-mediated disease later in life. Young children are also particularly susceptible to infections, warranting more analyses of immune system development early in life. Such analyses mostly have been performed in mouse models or human cord blood samples, but these cannot account for the complex environmental exposures influencing human newborns after birth. Here, we performed longitudinal analyses in 100 newborn children, sampled up to 4 times during their first 3 months of life. From 100 μL of blood, we analyze the development of 58 immune cell populations by mass cytometry and 267 plasma proteins by immunoassays, uncovering drastic changes not predictable from cord blood measurements but following a stereotypic pattern. Preterm and term children differ at birth but converge onto a shared trajectory, seemingly driven by microbial interactions and hampered by early gut bacterial dysbiosis. Cord blood is not representative of postnatal immunity Preterm and term children differ at birth but rapidly converge thereafter Immune system development follows a stereotypic pattern early in life Dynamic parameters imply microbial interactions during early immune development Longitudinal profiling of blood immune cells from 100 newborns provides a systemic view on the ontogeny of the human neonatal immune system.
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影响因子：
64.5
作者：
Brodin P;Jojic V;Gao T;Bhattacharya S;Angel CJ;Furman D;Shen-Orr S;Dekker CL;Swan GE;Butte AJ;Maecker HT;Davis MM
通讯作者：
Davis MM
影响因子：
17.1
作者：
Arrieta, Marie-Claire;Stiemsma, Leah T.;Finlay, B. Brett
通讯作者：
Finlay, B. Brett
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
82.9
作者：
Gibbons, Deena;Fleming, Paul;Hayday, Adrian
通讯作者：
Hayday, Adrian
影响因子：
5.4
作者：
Leiber, Anja;Schwarz, Julian;Gille, Christian
通讯作者：
Gille, Christian