Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples.
Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples.
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SARS-CoV-2结合（IgG，IgM，伊加）和人血清样本中和抗体的比较分析。
DOI：
10.1016/j.jim.2020.112937
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发表时间：
2021-03
影响因子：
2.2
通讯作者：
Montomoli E
中科院分区：
文献类型：
--
作者：
Mazzini L;Martinuzzi D;Hyseni I;Benincasa L;Molesti E;Casa E;Lapini G;Piu P;Trombetta CM;Marchi S;Razzano I;Manenti A;Montomoli E
一种新发现的冠状病毒，命名为SARS-CoV-2，于2019年1,2月在中国湖北省出现，并迅速蔓延到世界各地;到目前为止，它已造成超过4970万例疾病和120万例死亡。SARS-CoV-2感染的诊断目前基于通过基于分子的测定（例如实时RT-PCR）检测鼻咽拭子中的病毒RNA。此外，检测不同类别抗体的血清学测定构成了收集关于感染的体液免疫反应和病毒在人群中传播的信息的极好的监测策略。此外，它还有助于评估用于治疗和预防COVID-19疾病的新型未来疫苗和药物的免疫原性。本研究的目的是确定SARS-CoV-2特异性抗体在人类血清样品中通过不同的商业和内部的ELISA试剂盒，以评估和比较他们的结果首先与彼此，然后与功能检测使用野生型病毒。确定SARS-CoV-2特异性IgM、IgG和伊加抗体的水平对于预测人群免疫力、与其他冠状病毒可能的交叉反应性以及识别潜在的传染性受试者非常重要。此外，在一小组样本中，进行了亚型IgG ELISA。我们的研究结果表明，中和滴度和IgG，IgM和伊加ELISA反应对刺突蛋白的受体结合结构域之间有显着的统计相关性。从而证实，针对病毒刺突蛋白这一部分的抗体具有高度中和性，并且基于ELISA受体结合结构域的测定法可用作不具备生物安保3级设施的实验室中和测定法的有效替代方法。
A newly identified coronavirus, named SARS-CoV-2, emerged in December 2019 in Hubei Province, China, and quickly spread throughout the world; so far, it has caused more than 49.7 million cases of disease and 1,2 million deaths. The diagnosis of SARS-CoV-2 infection is currently based on the detection of viral RNA in nasopharyngeal swabs by means of molecular-based assays, such as real-time RT-PCR. Furthermore, serological assays detecting different classes of antibodies constitute an excellent surveillance strategy for gathering information on the humoral immune response to infection and the spread of the virus through the population. In addition, it can contribute to evaluate the immunogenicity of novel future vaccines and medicines for the treatment and prevention of COVID-19 disease. The aim of this study was to determine SARS-CoV-2-specific antibodies in human serum samples by means of different commercial and in-house ELISA kits, in order to evaluate and compare their results first with one another and then with those yielded by functional assays using wild-type virus. It is important to identify the level of SARS-CoV-2-specific IgM, IgG and IgA antibodies in order to predict human population immunity, possible cross-reactivity with other coronaviruses and to identify potentially infectious subjects. In addition, in a small sub-group of samples, a subtyping IgG ELISA has been performed. Our findings showed a notable statistical correlation between the neutralization titers and the IgG, IgM and IgA ELISA responses against the receptor-binding domain of the spike protein. Thus confirming that antibodies against this portion of the virus spike protein are highly neutralizing and that the ELISA Receptor-Binding Domain-based assay can be used as a valid surrogate for the neutralization assay in laboratories that do not have biosecurity level-3 facilities.
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影响因子：
5.5
作者：
Madore, Dace V.;Meade, Bruce D.;Rubin, Fran;Deal, Carolyn;Lynn, Freyja
通讯作者：
Lynn, Freyja
DOI：
10.1038/nrmicro.2016.81
发表时间：
2016-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
de Wit E;van Doremalen N;Falzarano D;Munster VJ
通讯作者：
Munster VJ
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
168.9
作者：
Chan, Jasper Fuk-Woo;Yuan, Shuofeng;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
3.1
作者：
Ivanov, Alexander P.;Klebleyeva, Tatiana D.;Ivanova, Olga E.
通讯作者：
Ivanova, Olga E.