Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration
Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration
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通过 bFGF 伪装的免疫调节电纺纤维支架用于骨盆再生
DOI：
10.1016/j.apmt.2019.04.005
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发表时间：
2019-06
影响因子：
8.3
通讯作者：
Cui Wenguo
中科院分区：
文献类型：
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作者：
Wang Yong;Cao Zhijuan;Cheng Ruoyu;Qin Menglu;Zhang D;an;Deng Lianfu;Chen Xinliang;Cui Wenguo
伤口愈合需要抗炎微环境和愈合级联反应，而从炎症阶段到增殖阶段的过渡是再生过程所必需的。然而，使用仿生材料可能会诱导局部慢性炎症，这可能会导致严重的感染和粘连，当它们调节炎症条件的能力尚未得到评估。为了加速伤口愈合过程，我们设想了一种支架，可以更好地调节免疫反应，同时减少炎症反应。在本研究中，除了独特的机械性能和较高的选择性蛋白质吸附率外，静电纺丝纤维还提供了抗炎微环境，并与天然细胞外基质的特征非常相似，可以通过其分子组分调节免疫细胞迁移，为伤口愈合提供更大的再生潜力。此外，我们还在聚乳酸（PLLA）表面接枝碱性成纤维细胞生长因子（bFGF），以提高材料的亲水性，保证材料的强度和生物相容性，并通过bFGF的持续释放影响免疫细胞因子，抑制炎症反应。此外，PLLA-bFGF表现出的细胞-材料相互作用模式促进人阴道成纤维细胞增殖，提高胶原基因的表达和蛋白质的分泌，包括COL-1/3，并调节免疫相关细胞因子的表达，包括转化生长因子β1和肿瘤坏死因子α。在兔腹壁疝体内模型中显示了相同的效果。总的来说，PLLA-bFGF支架可以通过其提供抗炎微环境和调节免疫调节因子的活性的特性来促进盆腔器官脱垂（POP）再生，并且因此可以高度实用地作为POP治疗中的经阴道修复的模板。
Wound healing requires an anti-inflammatory microenvironment and a healing cascade reaction, while transition from the inflammatory to the proliferative phase is necessary for the regeneration process. However, the use of biomimetic materials may induce local chronic inflammation, which could lead to serious infection and adhesion, when their ability to modulate inflammatory conditions has not been evaluated. To accelerate the wound-healing process, we envisioned a scaffold that can better regulate the immune response while reducing the inflammatory response. In the present study, in addition to the unique mechanical properties and higher rates of selective protein adsorption, electrospun fibers provided an anti-inflammatory microenvironment and closely resembled features of natural extracellular matrix, which could regulate immune-cell migration by its molecular component, providing greater regeneration potential in wound healing. Moreover, we grafted basic fibroblast growth factor (bFGF) on the surface of poly-l-lactic acid (PLLA) to improve the hydrophilicity, ensuring the strength and biocompatibility of the material, which could affect the immune cytokines and inhibit the inflammatory reaction through a constant bFGF release. Furthermore, the pattern of cell–material interaction exhibited by PLLA-bFGF promoted human vaginal fibroblast proliferation, improved the expression of collagen genes and secretion of the proteins, including COL-1/3, and regulated the expression of immune-related cytokines, including transforming growth factor β1 and tumor necrosis factor-α. The rabbit abdominal wall hernia in vivo model revealed the same effects. Overall, the PLLA-bFGF scaffold may promote pelvic organ prolapsed (POP) regeneration through its properties of providing an anti-inflammatory microenvironment and regulating the activities of immune-regulative factors, and may thus be highly practical as a template for transvaginal repair in POP treatment.
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影响因子：
1.8
作者：
Chen, BH;Wen, Y;Polan, ML
通讯作者：
Polan, ML
影响因子：
2
作者：
Roman, Sabiniano;Mangera, Altaf;MacNeil, Sheila
通讯作者：
MacNeil, Sheila
DOI：
10.1002/jbm.b.31234
发表时间：
2009-05-01
影响因子：
3.4
作者：
Bellon, J. M.;Rodriguez, M.;Bujan, J.
通讯作者：
Bujan, J.
DOI：
10.1016/j.colsurfb.2016.02.034
发表时间：
2016-06
期刊：
Colloids and surfaces. B, Biointerfaces
影响因子：
--
作者：
Dandan Zhang;Z. Lin;Ruoyu Cheng;Wei Wu;Jia Yu;Xin Zhao;Xinliang Chen;W. Cui
通讯作者：
Dandan Zhang;Z. Lin;Ruoyu Cheng;Wei Wu;Jia Yu;Xin Zhao;Xinliang Chen;W. Cui
影响因子：
7.2
作者：
通讯作者：
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