Species and genus level resolution analysis of gut microbiota in Clostridium difficile patients following fecal microbiota transplantation.
Species and genus level resolution analysis of gut microbiota in Clostridium difficile patients following fecal microbiota transplantation.
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DOI：
10.1186/2049-2618-2-13
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发表时间：
2014
期刊：
影响因子：
15.5
通讯作者：
Sadowsky MJ
中科院分区：
文献类型：
--
作者：
Shankar V;Hamilton MJ;Khoruts A;Kilburn A;Unno T;Paliy O;Sadowsky MJ
艰难梭菌是一种机会致病菌，艰难梭菌感染(CDI)是引起抗生素所致腹泻和结肠炎的主要原因之一。对抗CDI，特别是复发性CDI的一种成功方法是通过将健康捐赠者的粪便微生物群移植到受感染的患者身上。在这项研究中，我们调查了三名CDI患者在粪便微生物区系移植前后的远端肠道微生物群落，并将这些群落与捐赠者粪便微生物区系的组成进行了比较。我们利用系统发育微生物区系阵列、高通量Illumina测序和荧光原位杂交来描述微生物区系组成，直至属和种水平的分辨率。原始患者的微生物区系多样性较低，以革兰氏杆菌和芽孢杆菌为主，梭状芽胞杆菌和杆菌数量较少。在属水平上，CDI患者的粪便样本中含有丰富的乳杆菌、链球菌和肠杆菌属成员。相比之下，供体群落以梭状芽胞杆菌为主，具有明显较高的多样性和均匀度。患者的远端肠道群落在粪便移植后3天内完全改变，每个患者的这些群落至少在4个月内保持稳定。尽管受者治疗前肠道微生物区系的组成有所不同，但移植后受者之间的肠道群落高度相似，与供者的肠道群落没有区别，并且含有丰富的布鲁氏菌、柯普氏菌和链球菌。在每种情况下，肠道微生物区系的恢复都导致患者完全康复和症状缓解。我们的结论是，艰难梭菌感染可以通过粪便微生物区系移植成功地治疗，这导致远端肠道微生物群落从富含耐氧物种的微生物群落稳定地转变为以梭状芽胞杆菌成员为主的微生物群落。
Clostridium difficile is an opportunistic human intestinal pathogen, and C. difficile infection (CDI) is one of the main causes of antibiotic-induced diarrhea and colitis. One successful approach to combat CDI, particularly recurrent form of CDI, is through transplantation of fecal microbiota from a healthy donor to the infected patient. In this study we investigated the distal gut microbial communities of three CDI patients before and after fecal microbiota transplantation, and we compared these communities to the composition of the donor’s fecal microbiota. We utilized phylogenetic Microbiota Array, high-throughput Illumina sequencing, and fluorescent in situ hybridization to profile microbiota composition down to the genus and species level resolution. The original patients’ microbiota had low diversity, was dominated by members of Gammaproteobacteria and Bacilli, and had low numbers of Clostridia and Bacteroidia. At the genus level, fecal samples of CDI patients were rich in members of the Lactobacillus, Streptococcus, and Enterobacter genera. In comparison, the donor community was dominated by Clostridia and had significantly higher diversity and evenness. The patients’ distal gut communities were completely transformed within 3 days following fecal transplantation, and these communities remained stable in each patient for at least 4 months. Despite compositional differences among recipients’ pre-treatment gut microbiota, the transplanted gut communities were highly similar among recipients post-transplantation, were indistinguishable from that of the donor, and were rich in members of Blautia, Coprococcus, and Faecalibacterium. In each case, the gut microbiota restoration led to a complete patient recovery and symptom alleviation. We conclude that C. difficile infection can be successfully treated by fecal microbiota transplantation and that this leads to stable transformation of the distal gut microbial community from the one abundant in aerotolerant species to that dominated by members of the Clostridia.
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影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
2.9
作者：
Khoruts, Alexander;Dicksved, Johan;Sadowsky, Michael J.
通讯作者：
Sadowsky, Michael J.
影响因子：
5.9
作者：
Khardori, Nancy
通讯作者：
Khardori, Nancy
影响因子：
12.3
作者：
Caporaso JG;Lauber CL;Costello EK;Berg-Lyons D;Gonzalez A;Stombaugh J;Knights D;Gajer P;Ravel J;Fierer N;Gordon JI;Knight R
通讯作者：
Knight R
影响因子：
5.8
作者：
Paliy, Oleg;Foy, Brent D.
通讯作者：
Foy, Brent D.