Defining Functions of Mannoproteins in Saccharomyces cerevisiae by High-Dimensional Morphological Phenotyping.
Defining Functions of Mannoproteins in Saccharomyces cerevisiae by High-Dimensional Morphological Phenotyping.
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通过高维形态表型来定义酿酒酵母中甘露蛋白的功能。
DOI：
10.3390/jof7090769
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发表时间：
2021-09-17
期刊：
影响因子：
--
通讯作者：
Ohya Y
中科院分区：
文献类型：
--
作者：
Ghanegolmohammadi F;Okada H;Liu Y;Itto-Nakama K;Ohnuki S;Savchenko A;Bi E;Yoshida S;Ohya Y
甘露糖蛋白是位于酵母细胞壁最外层的非丝状糖蛋白。由于适当工具的有限性，这些结构成分的生理作用尚未完全阐明。由于甘露糖蛋白的扰动可能会影响细胞形态，我们通过高维形态表型研究了酿酒酵母中的甘露糖蛋白突变体。甘露糖蛋白突变体的形态学分为七组，使用高斯混合模型的聚类分析。簇I突变体细胞（ccw12Δ）的多效性表型表明CCW12在细胞壁组织中起主要作用。簇II（ccw14Δ、flo11Δ、srl1Δ和tir3Δ）突变体表现出改变的母细胞大小和形状。簇III和IV的突变体没有表现出或表现出非常小的形态缺陷。簇V（dse2Δ、egt2Δ和sun4Δ）由由于不完全隔膜消化而具有细胞分离缺陷的内切葡聚糖酶突变体组成。簇VI突变体细胞（ecm33Δ）表现出顶芽生长的扰动。簇VII突变细胞（sag1Δ）在细胞大小和肌动蛋白组织方面表现出差异。生化分析进一步证实了观察到的形态缺陷。基于各种组学数据的进一步研究表明，形态表型是一种补充工具，可以帮助更深入地了解甘露糖蛋白的功能。
Mannoproteins are non-filamentous glycoproteins localized to the outermost layer of the yeast cell wall. The physiological roles of these structural components have not been completely elucidated due to the limited availability of appropriate tools. As the perturbation of mannoproteins may affect cell morphology, we investigated mannoprotein mutants in Saccharomyces cerevisiae via high-dimensional morphological phenotyping. The mannoprotein mutants were morphologically classified into seven groups using clustering analysis with Gaussian mixture modeling. The pleiotropic phenotypes of cluster I mutant cells (ccw12Δ) indicated that CCW12 plays major roles in cell wall organization. Cluster II (ccw14Δ, flo11Δ, srl1Δ, and tir3Δ) mutants exhibited altered mother cell size and shape. Mutants of cluster III and IV exhibited no or very small morphological defects. Cluster V (dse2Δ, egt2Δ, and sun4Δ) consisted of endoglucanase mutants with cell separation defects due to incomplete septum digestion. The cluster VI mutant cells (ecm33Δ) exhibited perturbation of apical bud growth. Cluster VII mutant cells (sag1Δ) exhibited differences in cell size and actin organization. Biochemical assays further confirmed the observed morphological defects. Further investigations based on various omics data indicated that morphological phenotyping is a complementary tool that can help with gaining a deeper understanding of the functions of mannoproteins.
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DOI：
10.1083/jcb.201508119
发表时间：
2016-01-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jonasson EM;Rossio V;Hatakeyama R;Abe M;Ohya Y;Yoshida S
通讯作者：
Yoshida S
影响因子：
6
作者：
Chadani T;Ohnuki S;Isogai A;Goshima T;Kashima M;Ghanegolmohammadi F;Nishi T;Hirata D;Watanabe D;Kitamoto K;Akao T;Ohya Y
通讯作者：
Ohya Y
影响因子：
2.6
作者：
Gonzalez, Marlyn;Goddard, Noel;Hicks, Charles;Ovalle, Rafael;Rauceo, Jason M.;Jue, Chong K.;Lipke, Peter N.
通讯作者：
Lipke, Peter N.
影响因子：
48
作者：
Caicedo JC;Cooper S;Heigwer F;Warchal S;Qiu P;Molnar C;Vasilevich AS;Barry JD;Bansal HS;Kraus O;Wawer M;Paavolainen L;Herrmann MD;Rohban M;Hung J;Hennig H;Concannon J;Smith I;Clemons PA;Singh S;Rees P;Horvath P;Linington RG;Carpenter AE
通讯作者：
Carpenter AE
影响因子：
64.5
作者：
Colman-Lerner, A;Chin, TE;Brent, R
通讯作者：
Brent, R