Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure.
Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure.
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DOI：
10.1038/s41598-017-10735-x
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发表时间：
2017-08-31
影响因子：
4.6
通讯作者：
Biernaskie J
中科院分区：
文献类型：
--
作者：
Anjum F;Agabalyan NA;Sparks HD;Rosin NL;Kallos MS;Biernaskie J
以细胞为基础的治疗最近成为促进皮肤伤口愈合的许多研究的重点。一个重要的挑战将是开发细胞输送工具，以促进细胞在伤口床上的存活和均匀分布。这些系统应该足够坚硬，便于操作，同时足够柔软，以限制对新合成的伤口组织的损害，并将患者的不适降至最低。在这里，我们开发了几种新型的由聚(ε-己内酯)(PCL)和明胶(GE)组成的可修饰纳米纤维支架。我们询问它们是否可以用作成人皮肤来源的前体细胞(HSKPs)的功能容器，以及裸支架如何影响内源性皮肤伤口愈合。将PCL和Ge在单一易溶的溶剂中电纺制成复合支架，并显示出独特的形态和力学性能。在种植成人hSKPs后，细胞外基质蛋白和硫酸糖胺聚糖在复合移植物中的沉积被发现增加。此外，复合支架显示出显著更高的细胞增殖，更大的细胞铺展和在纳米纤维垫内的整合。将脱细胞支架移植到创面，显示支架在真皮-表皮厚度、轴突密度和胶原沉积方面有改善。这些结果表明，基于PCL的纳米纤维支架有望成为伤口愈合的细胞输送系统。
Cell-based therapies have recently been the focus of much research to enhance skin wound healing. An important challenge will be to develop vehicles for cell delivery that promote survival and uniform distribution of cells across the wound bed. These systems should be stiff enough to facilitate handling, whilst soft enough to limit damage to newly synthesized wound tissue and minimize patient discomfort. Herein, we developed several novel modifiable nanofibre scaffolds comprised of Poly (ε-caprolactone) (PCL) and gelatin (GE). We asked whether they could be used as a functional receptacle for adult human Skin-derived Precursor Cells (hSKPs) and how naked scaffolds impact endogenous skin wound healing. PCL and GE were electrospun in a single facile solvent to create composite scaffolds and displayed unique morphological and mechanical properties. After seeding with adult hSKPs, deposition of extracellular matrix proteins and sulphated glycosaminoglycans was found to be enhanced in composite grafts. Moreover, composite scaffolds exhibited significantly higher cell proliferation, greater cell spreading and integration within the nanofiber mats. Transplantation of acellular scaffolds into wounds revealed scaffolds exhibited improvement in dermal-epidermal thickness, axonal density and collagen deposition. These results demonstrate that PCL-based nanofiber scaffolds show promise as a cell delivery system for wound healing.
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