A Novel Competing Endogenous RNA Network Associated With the Pathogenesis of Graves' Ophthalmopathy.
A Novel Competing Endogenous RNA Network Associated With the Pathogenesis of Graves' Ophthalmopathy.
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一种与 Graves 眼病发病机制相关的新型竞争性内源 RNA 网络
DOI：
10.3389/fgene.2021.795546
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发表时间：
2021
影响因子：
3.7
通讯作者：
Wei R
中科院分区：
文献类型：
--
作者：
Yue Z;Mou P;Chen S;Tong F;Wei R
背景资料：最近越来越多的证据揭示了许多人类疾病中长非编码（lncRNA）/环状RNA（circRNA）-microRNA（miRNA）-mRNA网络的特征。然而，与Graves眼病（GO）相关的科学lncRNA/circRNA-miRNA-mRNA网络仍然缺乏。 材料与方法：通过高通量测序技术测定GO患者的RNA表达水平，并通过定量实时PCR（qPCR）验证结果。我们构建了一个蛋白质-蛋白质相互作用（PPI）网络使用检索工具的相互作用基因（STRING）数据库和确定枢纽基因的Cytoscape插件CytoHubba。通过种子序列匹配分析，预测差异表达的lncRNA/circRNA和mRNA相关的miRNAs。对感兴趣的RNA进行相关系数分析以构建新的竞争性内源RNA（ceRNA）网络。 结果如下：与对照组相比，GO患者中总共有361个mRNA、355个circRNA和242个lncRNA差异表达，鉴定了166对，并构建了ceRNA网络。qPCR结果显示，GO患者和对照患者之间存在4种mRNA（THBS 2、CHRM 3、CXCL 1、FPR 2）和2种lncRNA（LINC 01820：13、ENST 00000499452）的差异表达。 结论：构建了GO患者和对照患者之间的创新lncRNA/circRNA-miRNA-mRNA ceRNA网络，并鉴定了两条重要的ceRNA通路，LINC 01820：13-hsa-miR-27 b-3 p-FPR 2 ceRNA通路和ENST 00000499452-hsa-miR-27 a-3 p-CXCL 1通路，其可能影响GO患者的自身免疫反应和炎症。
Background: Growing evidence has recently revealed the characteristics of long noncoding (lncRNA)/circular RNA (circRNA)-microRNA (miRNA)-mRNA networks in numerous human diseases. However, a scientific lncRNA/circRNA-miRNA-mRNA network related to Graves’ ophthalmopathy (GO) remains lacking. Materials and methods: The expression levels of RNAs in GO patients were measured through high-throughput sequencing technology, and the results were proven by quantitative real-time PCR (qPCR). We constructed a protein-protein interaction (PPI) network using the Search Tool for the Retrieval of Interacting Genes (STRING) database and identified hub genes by the Cytoscape plug-in CytoHubba. Then, the miRNAs related to differentially expressed lncRNAs/circRNAs and mRNAs were predicted through seed sequence matching analysis. Correlation coefficient analysis was performed on the interesting RNAs to construct a novel competing endogenous RNA (ceRNA) network. Results: In total, 361 mRNAs, 355 circRNAs, and 242 lncRNAs were differentially expressed in GO patients compared with control patients, 166 pairs were identified, and ceRNA networks were constructed. The qPCR results showed that 4 mRNAs (THBS2, CHRM3, CXCL1, FPR2) and 2 lncRNAs (LINC01820:13, ENST00000499452) were differentially expressed between the GO patients and control patients. Conclusion: An innovative lncRNA/circRNA-miRNA-mRNA ceRNA network between GO patients and control patients was constructed, and two important ceRNA pathways were identified, the LINC01820:13-hsa-miR-27b-3p-FPR2 ceRNA pathway and the ENST00000499452-hsa-miR-27a-3p-CXCL1 pathway, which probably affect the autoimmune response and inflammation in GO patients.
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影响因子：
3.6
作者：
Ni S;Jiang T;Hao S;Luo P;Wang P;Almatari Y;Wang Y;Zhang Z;Guo L
通讯作者：
Guo L
影响因子：
28.2
作者：
Karreth FA;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
3.5
作者：
Bednarczuk, T;Kiljanski, J;Wall, JR
通讯作者：
Wall, JR
影响因子：
4.4
作者：
Ko, JaeSang;Kim, Ji-Young;Yoon, Jin Sook
通讯作者：
Yoon, Jin Sook
影响因子：
4.1
作者：
Jang, Sun Young;Park, Seong Jun;Yoon, Jin Sook
通讯作者：
Yoon, Jin Sook