Association of the gut microbiome and metabolome with wheeze frequency in childhood asthma.
Association of the gut microbiome and metabolome with wheeze frequency in childhood asthma.
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DOI：
10.1016/j.jaci.2022.02.005
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发表时间：
2022-08
影响因子：
14.2
通讯作者：
Weiss, Scott T.
中科院分区：
文献类型：
--
作者：
Lee-Sarwar, Kathleen;Dedrick, Sandra;Momeni, Babak;Kelly, Rachel S.;Zeiger, Robert S.;O'Connor, George T.;Sandel, Megan T.;Bacharier, Leonard B.;Beigelman, Avraham;Laranjo, Nancy;Gold, Diane R.;Lasky-Su, Jessica;Litonjua, Augusto A.;Liu, Yang-Yu;Weiss, Scott T.
虽然微生物组在哮喘发展中具有既定的作用，但对其对哮喘儿童发病率的贡献知之甚少。在这项维生素D减少哮喘试验（VDAART）的辅助研究中，我们分析了哮喘儿童喘息频率的肠道微生物组和代谢组。对从患有父母报告的医生诊断的哮喘的三岁儿童收集的粪便样本进行细菌16 s rRNA微生物组和非靶向代谢组学分析。我们通过计算3 - 5岁儿童每季度一次的问卷调查中父母报告孩子有喘息的比例（“喘息比例”）来分析喘息频率。通过确定喘息频率的对数倍数变化和相关性/线性回归分析，分别分析与喘息相关的分类和代谢物。比较喘息发生率高与低受试者的微生物-代谢物和微生物-微生物相关网络。特定的分类群，包括韦荣氏球菌属和组氨酸途径代谢产物，在喘息比例高的受试者中富集。在喘鸣相关的分类群中，韦荣球菌和颤螺菌UCG-005与喘鸣呈负相关，与粪便代谢物的数量最多。微生物网络在喘息频率低与高的受试者之间相似。肠道微生物组特征与哮喘儿童喘息频率相关，表明肠道微生物组对儿童哮喘发病率的影响。在哮喘儿童中，肠道中的特定细菌和小分子与喘息频率增加有关，这表明针对微生物组的策略可以降低儿童哮喘的发病率。
While the microbiome has an established role in asthma development, less is known about its contribution to morbidity in children with asthma. In this ancillary study of the Vitamin D Antenatal Asthma Reduction Trial (VDAART), we analyzed the gut microbiome and metabolome of wheeze frequency in children with asthma. Bacterial 16s rRNA microbiome and untargeted metabolomic profiling were performed on fecal samples collected from three-year-old children with parent-reported physician-diagnosed asthma. We analyzed wheeze frequency by calculating the proportion of quarterly questionnaires between ages 3 and 5 years in which parents reported the child had wheezed (“wheeze proportion”). Taxa and metabolites associated with wheeze were analyzed by identifying log-fold changes with respect to wheeze frequency and correlation/linear regression analyses, respectively. Microbe-metabolite and microbe-microbe correlation networks were compared between subjects with high and low wheeze proportion. Specific taxa, including the genus Veillonella and histidine pathway metabolites, were enriched in subjects with high wheeze proportion. Amongst wheeze-associated taxa, Veillonella and Oscillospiraceae UCG-005, which was inversely associated with wheeze, were correlated with the greatest number of fecal metabolites. Microbial networks were similar between subjects with low vs. high wheeze frequency. Gut microbiome features are associated with wheeze frequency in children with asthma, suggesting an impact of the gut microbiome on morbidity in childhood asthma. In children with asthma, specific bacteria and small molecules in the gut are associated with increased frequency of wheeze, suggesting that strategies that target the microbiome could reduce morbidity in childhood asthma.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Huang YJ;Nelson CE;Brodie EL;Desantis TZ;Baek MS;Liu J;Woyke T;Allgaier M;Bristow J;Wiener-Kronish JP;Sutherland ER;King TS;Icitovic N;Martin RJ;Calhoun WJ;Castro M;Denlinger LC;Dimango E;Kraft M;Peters SP;Wasserman SI;Wechsler ME;Boushey HA;Lynch SV;National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
影响因子：
4.3
作者：
Faust K;Sathirapongsasuti JF;Izard J;Segata N;Gevers D;Raes J;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
17.1
作者：
Arrieta, Marie-Claire;Stiemsma, Leah T.;Finlay, B. Brett
通讯作者：
Finlay, B. Brett
影响因子：
8.4
作者：
Chauhan, Bhupendrasinh F.;Ducharmc, Francine M.
通讯作者：
Ducharmc, Francine M.