Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor
Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor
复制标题
具有受控释放碱性成纤维细胞生长因子的肝素功能化胶原基质
DOI：
10.1007/s10856-010-4176-4
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Guo Y
中科院分区：
文献类型：
--
作者：
Wu JM;Li RX;Zhang H;Xu YY;Li ZH;Yuan XY;Wang PF;Zhang XZ;Liu YQ;Guan J;Guo Y
Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified byN-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) andN-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold.
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影响因子：
2.9
作者：
K. Kamei;X. Wu;X. Xu;K. Minami;N. Huy;R. Takano;H. Kato;S. Hara
通讯作者：
K. Kamei;X. Wu;X. Xu;K. Minami;N. Huy;R. Takano;H. Kato;S. Hara
影响因子：
14
作者：
Jeon, O;Kang, SW;Kim, BS
通讯作者：
Kim, BS
DOI：
10.1016/s0021-9258(19)37085-1
发表时间：
1992-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Salmivirta;J. Heino;M. Jalkanen
通讯作者：
M. Salmivirta;J. Heino;M. Jalkanen
DOI：
10.1016/s1357-2725(99)00123-5
发表时间：
2000-02-01
影响因子：
4
作者：
Nugent, MA;Iozzo, RV
通讯作者：
Iozzo, RV
影响因子：
14
作者：
Tabata, Y;Nagano, A;Ikada, Y
通讯作者：
Ikada, Y