Novel epigenetic changes unveiled by monozygotic twins discordant for smoking habits.
Novel epigenetic changes unveiled by monozygotic twins discordant for smoking habits.
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DOI：
10.1371/journal.pone.0128265
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Matullo G
中科院分区：
文献类型：
--
作者：
Allione A;Marcon F;Fiorito G;Guarrera S;Siniscalchi E;Zijno A;Crebelli R;Matullo G
吸烟会影响表观基因组，并可能增加患癌症和心血管疾病等疾病的风险。与吸烟相关的DNA甲基化的变化可能有助于确定导致疾病病因的分子途径。以前的研究在识别吸烟者DNA中的差异甲基化区域方面并不完全一致。我们在一组吸烟习惯不一致的单卵（MZ）双胞胎中进行了表观基因组范围的DNA甲基化研究，以确定吸烟对DNA甲基化的影响。由于MZ双胞胎被认为在遗传上是相同的，这个模型使我们能够识别与吸烟相关的DNA甲基化变化，而不依赖于遗传成分。我们使用Illumina HumanMethylation450微珠芯片研究了20对吸烟习惯不一致的同卵双胞胎的全血全基因组DNA甲基化谱。我们通过对内分析确定了吸烟者和非吸烟者MZ双胞胎之间甲基化差异的22个CpG位点。我们在AHRR、F2RL3、MYOG 1基因2q37.1和6p21.33区域确认了8个已被其他研究组描述的基因座，并鉴定了几个新的基因座。此外，途径分析显示参与GTdR调节活性的基因的富集。我们的研究证实了吸烟相关的DNA甲基化变化的证据，强调设计良好的MZ双胞胎模型可以帮助发现新的DNA甲基化信号，即使在有限的样本人群中。
Exposure to cigarette smoking affects the epigenome and could increase the risk of developing diseases such as cancer and cardiovascular disorders. Changes in DNA methylation associated with smoking may help to identify molecular pathways that contribute to disease etiology. Previous studies are not completely concordant in the identification of differentially methylated regions in the DNA of smokers. We performed an epigenome-wide DNA methylation study in a group of monozygotic (MZ) twins discordant for smoking habits to determine the effect of smoking on DNA methylation. As MZ twins are considered genetically identical, this model allowed us to identify smoking-related DNA methylation changes independent from genetic components. We investigated the whole blood genome-wide DNA methylation profiles in 20 MZ twin pairs discordant for smoking habits by using the Illumina HumanMethylation450 BeadChip. We identified 22 CpG sites that were differentially methylated between smoker and non-smoker MZ twins by intra-pair analysis. We confirmed eight loci already described by other groups, located in AHRR, F2RL3, MYOG1 genes, at 2q37.1 and 6p21.33 regions, and also identified several new loci. Moreover, pathway analysis showed an enrichment of genes involved in GTPase regulatory activity. Our study confirmed the evidence of smoking-related DNA methylation changes, emphasizing that well-designed MZ twin models can aid the discovery of novel DNA methylation signals, even in a limited sample population.
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影响因子：
11.5
作者：
Belinsky, SA;Klinge, DM;Picchi, MA
通讯作者：
Picchi, MA
乳腺癌不一致同卵双胞胎的 DNA 甲基化分析将 DOK7 确定为新型表观遗传生物标志物。
影响因子：
4.7
作者：
Heyn H;Carmona FJ;Gomez A;Ferreira HJ;Bell JT;Sayols S;Ward K;Stefansson OA;Moran S;Sandoval J;Eyfjord JE;Spector TD;Esteller M
通讯作者：
Esteller M
影响因子：
3.5
作者：
Besingi, Welisane;Johansson, Asa
通讯作者：
Johansson, Asa
DOI：
10.1164/rccm.201110-1868oc
发表时间：
2012-10-01
影响因子：
24.7
作者：
Lange, Nancy E.;Sparrow, David;Litonjua, Augusto A.
通讯作者：
Litonjua, Augusto A.
影响因子：
6.8
作者：
Andreoli, Cristina;Gregg, Evan O.;Bassi, Antonella
通讯作者：
Bassi, Antonella