The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation.
The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation.
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DOI：
10.1083/jcb.200810130
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发表时间：
2009-04-20
期刊：
影响因子：
--
通讯作者：
Jayaram M
中科院分区：
文献类型：
--
作者：
Cui H;Ghosh SK;Jayaram M
酿酒酵母的2微米质粒使用Kip1马达，而不是功能冗余的Cin8马达，因为其精确的核定位和均匀的分离。Kip1p与质粒区STB结合的时间和寿命与Kip1p是质粒区复合体的真实成分相一致。Kip1-STB的结合不会因为拆解有丝分裂纺锤体而被阻断。Kip1p的缺失扰乱了粘附素复合体在STB的招募和复制的质粒分子的凝聚力。2微米报告质粒与Kip1p在纺锤体极体附近的共存使人想起CEN报告质粒。Kip1p的缺失取代了这个核地址的质粒，在那里它有可能拴在染色体上或窃取染色体分离因子。利用Kip1p，它是Cin8p的副产品，用于染色体分离，将自己定向到“分割中心”，这代表了酵母质粒良性寄生的另一个方面。
The 2 micron plasmid of Saccharomyces cerevisiae uses the Kip1 motor, but not the functionally redundant Cin8 motor, for its precise nuclear localization and equal segregation. The timing and lifetime of Kip1p association with the plasmid partitioning locus STB are consistent with Kip1p being an authentic component of the plasmid partitioning complex. Kip1–STB association is not blocked by disassembling the mitotic spindle. Lack of Kip1p disrupts recruitment of the cohesin complex at STB and cohesion of replicated plasmid molecules. Colocalization of a 2 micron reporter plasmid with Kip1p in close proximity to the spindle pole body is reminiscent of that of a CEN reporter plasmid. Absence of Kip1p displaces the plasmid from this nuclear address, where it has the potential to tether to a chromosome or poach chromosome segregation factors. Exploiting Kip1p, which is subsidiary to Cin8p for chromosome segregation, to direct itself to a “partitioning center” represents yet another facet of the benign parasitism of the yeast plasmid.
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影响因子：
16
作者：
Huang, J;Hsu, JM;Laurent, BC
通讯作者：
Laurent, BC
DOI：
10.1083/jcb.118.1.109
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
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作者：
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作者：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.231212498
发表时间：
2001-10-23
影响因子：
11.1
作者：
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通讯作者：
Roof, DM