Effects of disinfectants against norovirus virus‐like particles predict norovirus inactivation
Effects of disinfectants against norovirus virus‐like particles predict norovirus inactivation
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消毒剂对诺如病毒病毒样颗粒的作用可预测诺如病毒失活
DOI：
10.1111/1348-0421.12435
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发表时间：
2016
影响因子：
2.6
通讯作者：
K. Katayama
中科院分区：
文献类型：
--
作者：
Jun Sato;Motohiro Miki;H. Kubota;J. Hitomi;H. Tokuda;Reiko Todaka;K. Katayama
人类诺如病毒(NOV)是世界范围内流行性和散发性急性胃肠炎的主要原因。公共卫生和个人卫生是预防新城疫传播的重要措施之一。然而，目前还没有一种实用的新城疫细胞培养体系，已经用替代病毒对抗新城疫消毒剂和消毒剂的杀灭病毒效果进行了初步测试。本研究将新冠病毒样颗粒(VLP)作为新冠病毒的替代物，建立了一种评价新冠病毒灭活效果的方法。这种方法是基于次氯酸钠处理后血管内皮细胞的形态变化。VLP样品被发现以浓度依赖的方式变形和降解。在此基础上，根据形态变化和颗粒数量的不同，将次氯酸钠对VLP的影响分为四个阶段。利用所建立的标准，评估了乙醇、碳酸盐和碱溶液对VLP的疗效。用这些消毒剂在特定条件下处理后，观察VLP的变形和聚集。为了确定VLP的降解机理(S)，用次氯酸钠和乙醇处理后，用十二烷基硫酸钠-PAGE和免疫印迹法检测VLP。经500 ppm以上的次氯酸钠处理后，未检测到与主要衣壳蛋白Vp1相对应的条带，但经乙醇处理后仍有条带。这些结果表明，VLP作为NOV的替代标志物具有很好的潜力，可用于初步的杀毒效果试验，以确定化学制剂对NOV的作用机制(S)。
Human noroviruses (NoVs) are a major cause of epidemic and sporadic acute gastroenteritis worldwide. Public and personal hygiene is one of the most important countermeasures for preventing spread of NoV infection. However, no a practicable cell culture system for NoV had been developed, initial tests of the virucidal effectiveness of anti‐NoV disinfectants and sanitizers have been performed using surrogate viruses. In this study, NoV virus‐like particles (VLPs) were used as a new surrogate for NoVs and a method for evaluating NoV inactivation using them developed. This method is based on morphological changes in VLPs after treatment with sodium hypochlorite. VLP specimens were found to become deformed and degraded in a concentration‐dependent manner. Based on these results, the effects of sodium hypochlorite on VLPs were classified into four phases according to morphological changes and number of particles. Using the criteria thus established, the efficacy of ethanol, carbonates and alkali solutions against VLPs was evaluated. Deformation and aggregation of VLPs were observed after treatment with these disinfectants under specific conditions. To determine the degradation mechanism(s), VLPs were examined by SDS‐PAGE and immunoblotting after treatment with sodium hypochlorite and ethanol. The band corresponding to the major capsid protein, VP1, was not detected after treatment with sodium hypochlorite at concentrations greater than 500 ppm, but remained after treatment with ethanol. These results suggest that VLPs have excellent potential as a surrogate marker for NoVs and can be used in initial virucidal effectiveness tests to determine the mechanism(s) of chemical agents on NoVs.
影响因子：
4.4
作者：
H. Urakami;K. Ikarashi;K. Okamoto;Yukari Abe;Tamami Ikarashi;T. Kono;Y. Konagaya;N. Tanaka
通讯作者：
H. Urakami;K. Ikarashi;K. Okamoto;Yukari Abe;Tamami Ikarashi;T. Kono;Y. Konagaya;N. Tanaka
影响因子：
3.8
作者：
Hansman, GS;Natori, K;Takeda, N
通讯作者：
Takeda, N