Characterization of the effects of aryl-azido compounds and UVA irradiation on the viral proteins and infectivity of human immunodeficiency virus type 1.
Characterization of the effects of aryl-azido compounds and UVA irradiation on the viral proteins and infectivity of human immunodeficiency virus type 1.
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DOI：
10.1111/j.1751-1097.2010.00780.x
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发表时间：
2010-09
影响因子：
3.3
通讯作者：
Blumenthal R
中科院分区：
文献类型：
--
作者：
Belanger JM;Raviv Y;Viard M;Jason de la Cruz M;Nagashima K;Blumenthal R
Hydrophobic UV-activatable compounds have been shown to partition into the hydrophobic region of biological membranes to selectively label transmembrane proteins, and to inactivate enveloped viruses. Here, we analyze various UV-activatable azido- and iodo- based hydrophobic compounds for their ability to inactivate a model enveloped virus, human immunodeficiency virus (HIV-1 MN). Treatment of HIV-1 with 1,5-diazidonapthalene (DAN), 1-iodo, 5-azidonaphthalene (INA), 1-azidonaphthalene (AzNAP) or 4,4’-diazidobiphenyl (DABIPH) followed by UVA irradiation for 2 minutes, resulted in complete viral inactivation, whereas treatment using analogous non-azido containing controls had no effect. Incorporation of an azido moiety within these hydrophobic compounds to promote photoinduced covalent reactions with proteins was found to be the primary mechanism of viral inactivation for this class of compounds. Prolonged UVA irradiation of the virus in the presence of these azido compounds resulted in further modifications of viral proteins, due to the generation of reactive oxygen species, leading to aggregation as visualized via western blot analysis, providing additional viral modifications that may inhibit viral infectivity. Furthermore, inactivation using these compounds resulted in the preservation of surface antigenic structures (recognized by neutralizing antibodies b12, 2g12 and 4e10), which is favorable for the creation of vaccines from these inactivated virus preparations.
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DOI：
10.1006/bbrc.1998.9060
发表时间：
1998-07-30
影响因子：
3.1
作者：
Mariner, JM;McMahon, JB;Boyd, MR
通讯作者：
Boyd, MR
DOI：
10.1007/bf02019460
发表时间：
1983-01-01
影响因子：
4.5
作者：
ADLERSTORTHZ, K;SEHULSTER, LM;MELNICK, JL
通讯作者：
MELNICK, JL
影响因子：
3.4
作者：
Meiklejohn, BI;Rahman, NA;Barisas, BG
通讯作者：
Barisas, BG
影响因子：
2.9
作者：
BERCOVICI, T;GITLER, C
通讯作者：
GITLER, C
DOI：
10.1016/j.bbrc.2007.04.115
发表时间：
2007-06-29
影响因子：
3.1
作者：
Sharma, Anuj;Raviv, Yossef;Maheshwari, Radha K.
通讯作者：
Maheshwari, Radha K.