"Life-like" assessment of antimicrobial surfaces by a new touch transfer assay displays strong superiority of a copper alloy compared to silver containing surfaces.
"Life-like" assessment of antimicrobial surfaces by a new touch transfer assay displays strong superiority of a copper alloy compared to silver containing surfaces.
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DOI：
10.1371/journal.pone.0187442
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Wuske T
中科院分区：
文献类型：
--
作者：
Knobloch JK;Tofern S;Kunz W;Schütze S;Riecke M;Solbach W;Wuske T
在医疗保健相关环境中，细菌从无生命表面传播是医院获得性感染的重要来源。许多市售的医疗器械承诺实现抗菌活性以减少环境污染。在这项研究中，我们开发了一种触摸转移试验建模指纹传输调查表面的抗菌活性，与确认抗菌活性的修改后的ISO 22196（JIS Z 2801）测定，以测试这些表面在更现实的条件下。用消毒的手指或覆盖有无菌和湿润的棉手套的手指从干燥的标准化主要污染表面（PCS）上采集细菌。随后，通过在具有或不具有潜在抗菌活性的二次污染表面（SCS）上按压来转移细菌，并在24 h后测定相对减少率。使用湿润的无菌手套观察到PCS和SCS之间的稳定传播率。含铜合金显示出细菌负荷的至少十倍降低，始终达到小于2.5cfu/cm 2。相比之下，在接触转移测定中没有观察到含银表面和成熟的纯银银的细菌污染的显著减少。通过接触转移试验，我们成功地建立了一种新的可重复的交叉污染建模方法。使用新方法，我们能够证明，在修改后的ISO 22196（JIS Z 2801）测定中确认抗菌活性的几个表面在规定的环境条件下缺乏有效性。该数据表明，在声称与人体皮肤接触的干燥表面污染环境中的表面抗菌活性之前，应严格审查ISO 22196等基于液体的测定。我们建议新开发的触摸转移试验作为一个新的额外的工具，用于评估潜在的抗菌表面之前，在医院环境中使用。
Transmission of bacteria from inanimate surfaces in healthcare associated environments is an important source of hospital acquired infections. A number of commercially available medical devices promise to fulfill antibacterial activity to reduce environmental contamination. In this study we developed a touch transfer assay modeling fingerprint transmission to investigate the antibacterial activity of surfaces, with confirmed antibacterial activity by a modified ISO 22196 (JIS Z 2801) assay to test such surfaces under more realistic conditions. Bacteria were taken up from a dry standardized primary contaminated surface (PCS) with disinfected fingers or fingers covered with sterile and moistened cotton gloves. Subsequently, bacteria were transferred by pressing on secondary contaminated surfaces (SCS) with or without potential antibacterial activity and the relative reduction rate was determined after 24 h. A stable transmission rate between PCS and SCS was observed using moistened sterile gloves. A copper containing alloy displayed at least a tenfold reduction of the bacterial load consistently reaching less than 2.5 cfu/cm2. In contrast, no significant reduction of bacterial contamination by silver containing surfaces and matured pure silver was observed in the touch transfer assay. With the touch transfer assay we successfully established a new reproducible method modeling cross contamination. Using the new method we were able to demonstrate that several surfaces with confirmed antimicrobial activity in a modified ISO 22196 (JIS Z 2801) assay lacked effectiveness under defined ambient conditions. This data indicate that liquid based assays like the ISO 22196 should be critically reviewed before claiming antibacterial activity for surfaces in the setting of contamination of dry surfaces by contact to the human skin. We suggest the newly developed touch transfer assay as a new additional tool for the assessment of potential antimicrobial surfaces prior utilization in hospital environments.
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影响因子：
4.9
作者：
Otter, Jonathan A.;Yezli, Saber;French, Gary L.
通讯作者：
French, Gary L.
影响因子：
4.9
作者：
Knobloch, JKM;Jäger, S;Mack, D
通讯作者：
Mack, D
影响因子：
4.9
作者：
Malik, RE;Cooper, RA;Griffith, CJ
通讯作者：
Griffith, CJ
DOI：
10.1016/j.jhin.2003.09.017
发表时间：
2004-01
期刊：
The Journal of hospital infection
影响因子：
--
作者：
Dancer SJ
通讯作者：
Dancer SJ
影响因子：
11.8
作者：
Humphreys, Hilary
通讯作者：
Humphreys, Hilary