Sodium butyrate reverses lipopolysaccharide-induced mitochondrial dysfunction in lymphoblasts.
Sodium butyrate reverses lipopolysaccharide-induced mitochondrial dysfunction in lymphoblasts.
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DOI：
10.1111/jcmm.17342
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发表时间：
2022-06
影响因子：
5.3
通讯作者：
Wallace, Douglas C.
中科院分区：
文献类型：
--
作者：
Weiss, Scott L.;Zhang, Donglan;Farooqi, Sumera;Wallace, Douglas C.
丁酸是一种短链脂肪酸，由肠道微生物组内的微生物通过膳食纤维发酵产生。微生物衍生的丁酸盐已被证明可以促进免疫和代谢稳态，部分原因是其对线粒体功能的有益作用，因此已被提出作为一种可能的抗炎治疗。我们测试了丁酸盐可以减轻脓毒症条件下免疫细胞线粒体呼吸减少的假设，作为更好地理解丁酸盐作为脓毒症新疗法的潜力的初步步骤。在暴露于对照培养基或100 ng/ml脂多糖（LPS）的4种EB病毒转化的淋巴母细胞（LB）细胞系中比较线粒体呼吸和含量（以柠檬酸合酶活性测量）。LB与LPS +丁酸盐的共孵育和LPS刺激后用丁酸盐处理都逆转了在暴露于LPS而不含丁酸盐的LB中观察到的线粒体呼吸降低。无论是LPS，也没有丁酸导致柠檬酸合酶活性的显着变化。初步研究结果支持进一步研究丁酸盐可能有助于减轻脓毒症中的免疫炎症反应的潜在线粒体机制。
Butyrate is a short‐chain fatty acid that is produced by commensal microbes within the intestinal microbiome through fermentation of dietary fibre. Microbial‐derived butyrate has been shown to promote immunologic and metabolic homeostasis, in part through its beneficial effects on mitochondrial function, and thus has been proposed as a possible anti‐inflammatory therapy. We tested the hypothesis that butyrate could mitigate the decrease in mitochondrial respiration in immune cells under septic conditions as a preliminary step towards better understanding the potential for butyrate as a novel therapy in sepsis. Mitochondrial respiration and content (measured as citrate synthase activity) were compared within four Epstein–Barr virus‐transformed lymphoblast (LB) cell lines exposed to either control media or lipopolysaccharide (LPS) 100 ng/ml. Both co‐incubation of LBs with LPS + butyrate and treatment with butyrate after LPS stimulation reversed the decrease in mitochondrial respiration observed in LBs exposed to LPS without butyrate. Neither LPS nor butyrate led to significant changes in citrate synthase activity. The preliminary findings support further investigation of a potential mitochondrial‐based mechanism through which butyrate may help to mitigate the immuno‐inflammatory response in sepsis.
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影响因子：
29
作者：
Donohoe DR;Garge N;Zhang X;Sun W;O'Connell TM;Bunger MK;Bultman SJ
通讯作者：
Bultman SJ
影响因子：
3.1
作者：
Weiss, Scott L.;Zhang, Donglan;Wallace, Douglas C.
通讯作者：
Wallace, Douglas C.
影响因子：
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影响因子：
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作者：
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通讯作者：
MACFARLANE, GT
影响因子：
7.7
作者：
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通讯作者：
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