Gene expression profiling of the olfactory tissues of sex-separated and sex-combined female and male mice.
Gene expression profiling of the olfactory tissues of sex-separated and sex-combined female and male mice.
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DOI：
10.1038/sdata.2018.260
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发表时间：
2018-12-04
期刊：
影响因子：
9.8
通讯作者：
Jakob S
中科院分区：
文献类型：
--
作者：
Santoro SW;Jakob S
嗅觉经验可以改变小鼠嗅感觉上皮内化学感觉神经元的分子和细胞组成。我们试图调查小鼠嗅觉系统中细胞和分子变化的范围，作为其暴露于复杂和显著的气味集的功能：那些来自异性的气味。我们将小鼠与异性分开饲养（性别分离）或与异性一起饲养（性别结合），直到6个月大，结果产生了四组小鼠。取每只小鼠的主嗅上皮（MOE）、颏鼻器官（VNO）和嗅球（OB），提取rna。总共36个RNA样本，代表每个性别/条件/组织组合的三个生物重复，进行完整性分析，并用于制备RNA-seq文库，随后通过qPCR分析组织或性别特异性标记的存在。对文库进行配对端测序，每个重复约2000万个片段，并使用Tuxedo套件分析数据。
Olfactory experience can alter the molecular and cellular composition of chemosensory neurons within the olfactory sensory epithelia of mice. We sought to investigate the scope of cellular and molecular changes within a mouse’s olfactory system as a function of its exposure to complex and salient sets of odors: those emitted from members of the opposite sex. We housed mice either separated from members of the opposite sex (sex-separated) or together with members of the opposite sex (sex-combined) until six months of age, resulting in the generation of four cohorts of mice. From each mouse, the main olfactory epithelium (MOE), vomeronasal organ (VNO), and olfactory bulb (OB) were removed and RNA-extracted. A total of 36 RNA samples, representing three biological replicates per sex/condition/tissue combination, were analyzed for integrity and used to prepare RNA-seq libraries, which were subsequently analyzed via qPCR for the presence of tissue- or sex-specific markers. Libraries were paired-end sequenced to a depth of ~20 million fragments per replicate and the data were analyzed using the Tuxedo suite.
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