Microwave-Assisted Extraction of Cannabinoids in Hemp Nut Using Response Surface Methodology: Optimization and Comparative Study.
Microwave-Assisted Extraction of Cannabinoids in Hemp Nut Using Response Surface Methodology: Optimization and Comparative Study.
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DOI：
10.3390/molecules22111894
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发表时间：
2017-11-03
期刊：
影响因子：
--
通讯作者：
Wu YT
中科院分区：
文献类型：
--
作者：
Chang CW;Yen CC;Wu MT;Hsu MC;Wu YT
大麻坚果通常被纳入几种食品制剂中;然而，大多数国家根据大麻素含量对大麻产品制定法规。在这项研究中，我们开发了一种有效的微波辅助提取（MAE）大麻素的方法（即，大麻坚果中的Δ9-四氢大麻酚、大麻二酚和大麻酚。通过单因素试验和响应面分析法对微波辅助萃取工艺进行优化。还进行了比较研究，以确定MAE和参考提取方法，即热回流提取（HRE），索氏提取（SE），超临界流体提取（SFE）和超声波辅助提取（UAE）之间的提取率和形态的差异。在响应面分析的自变量中，温度是最显著的参数。结果表明，微波辅助提取火麻仁中大麻素的最佳工艺条件为：提取溶剂为甲醇，微波功率为375 W，提取温度为109 °C，提取时间为30 min（MAE为6.09 μg/g; HRE为4.15 μg/g; SE为5.81 μg/g; SFE为3.61 μg/g; UAE为3.73 μg/g），溶剂消耗量最少，时间最短。形态学观察表明，大量的细胞破裂后发生在微结构的大麻坚果MAE，表明在提取过程中的目标化合物的溶解增强。因此，MAE方法是一种快速、经济和环境友好的提取方法，对于工业应用来说既有效又实用。
Hemp nut is commonly incorporated into several food preparations; however, most countries set regulations for hemp products according to their cannabinoid content. In this study, we have developed an efficient microwave-assisted extraction (MAE) method for cannabinoids (i.e., Δ9-tetrahydrocannabinol, cannabidiol, and cannabinol) in hemp nut. Optimization of the MAE procedure was conducted through single factor experiments and response surface methodology (RSM). A comparative study was also conducted to determine the differences in the extraction yields and morphology of hemp nut between MAE and reference extraction methods, namely heat reflux extraction (HRE), Soxhlet extraction (SE), supercritical fluid extraction (SFE), and ultrasound-assisted extraction (UAE). Among the independent variables in RSM, the temperature was the most significant parameter. The optimal conditions of MAE were as follows: extraction solvent of methanol, microwave power of 375 W, temperature of 109 °C, and extraction time of 30 min. Compared with reference extraction methods, MAE achieved the highest extraction yields of total cannabinoids in hemp nut (6.09 μg/g for MAE; 4.15 μg/g for HRE; 5.81 μg/g for SE; 3.61 μg/g for SFE; 3.73 μg/g for UAE) with the least solvent consumption and shortest time. Morphological observations showed that substantial cell rupturing occurred in the microstructure of hemp nut after MAE, indicating enhanced dissolution of the target compounds during the extraction process. The MAE method is thus a rapid, economic, and environmentally friendly extraction method that is both effective and practical for industrial applications.
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DOI：
10.1016/j.jchromb.2009.11.004
发表时间：
2009-12-15
影响因子：
3
作者：
De Backer, Benjamin;Debrus, Benjamin;Charlier, Corinne
通讯作者：
Charlier, Corinne
影响因子：
1.9
作者：
Mariotti, Kristiane de Cassia;Alexandre Marcelo, Marcelo Caetano;Limberger, Renata Pereira
通讯作者：
Limberger, Renata Pereira
影响因子：
3.7
作者：
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通讯作者：
Luque de Castro, Maria D.
影响因子：
8.8
作者：
Bae, In Kyung;Ham, Hyeon Mi;Kim, Ho Jin
通讯作者：
Kim, Ho Jin
影响因子：
6.7
作者：
Abrams, D. I.;Guzman, M.
通讯作者：
Guzman, M.