A Role for Astroglial Calcium in Mammalian Sleep and Sleep Regulation.
A Role for Astroglial Calcium in Mammalian Sleep and Sleep Regulation.
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星形胶质细胞钙在哺乳动物睡眠和睡眠调节中的作用。
DOI：
10.1016/j.cub.2020.08.052
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发表时间：
2020-11-16
期刊：
影响因子：
--
通讯作者：
Frank MG
中科院分区：
文献类型：
--
作者：
Ingiosi AM;Hayworth CR;Harvey DO;Singletary KG;Rempe MJ;Wisor JP;Frank MG
哺乳动物的睡眠表达和调节历来被认为反映了神经元的活动。其他脑细胞（神经胶质细胞）在睡眠-觉醒周期中的变化及其在睡眠调节中的作用相对未被探索。我们发现，睡眠和觉醒伴随着状态依赖性的变化，星形胶质细胞的活动。使用微型显微镜在自由行为的小鼠和双光子显微镜在头部固定，未麻醉的小鼠，我们表明，星形胶质细胞钙信号是最高的清醒和最低的睡眠，是最明显的星形胶质细胞的过程。我们还发现，在非快速眼动睡眠期间，星形胶质细胞钙信号的变化与睡眠需求成比例。与神经元相比，星形胶质细胞在网络和单细胞水平上在睡眠剥夺后的非快速眼动睡眠期间变得不那么同步。最后，我们表明，有条件地减少星形胶质细胞内钙损害睡眠剥夺的稳态反应。因此，星形胶质细胞的钙活性在不同的警觉状态下动态变化，与睡眠需求成正比，是睡眠稳态的一个组成部分。Ingiosi等人使用体内成像方法描述了星形胶质细胞Ca 2+信号如何在睡眠和清醒期间以及睡眠丧失后动态变化。与清醒和睡眠剥夺后相比，睡眠期间星形胶质细胞Ca 2+信号的同步性降低。STIM 1对星形胶质细胞Ca 2+的调节在睡眠丧失的正常代偿反应中起作用。
Mammalian sleep expression and regulation has historically been thought to reflect the activity of neurons. Changes in other brain cells (glia) across the sleep-wake cycle and their role in sleep regulation are comparatively unexplored. We show that sleep and wakefulness are accompanied by state-dependent changes in astroglial activity. Using a miniature microscope in freely-behaving mice and a two-photon microscope in head-fixed, unanesthetized mice, we show that astroglial calcium signals are highest in wake and lowest in sleep and are most pronounced in astroglial processes. We also find that astroglial calcium signals during non-rapid eye movement sleep change in proportion to sleep need. In contrast to neurons, astrocytes become less synchronized during non-rapid eye movement sleep after sleep deprivation at the network and single cell level. Finally, we show that conditionally reducing intracellular calcium in astrocytes impairs the homeostatic response to sleep deprivation. Thus, astroglial calcium activity changes dynamically across vigilance states, is proportional to sleep need, and is a component of the sleep homeostat. Ingiosi et al. describe how astroglial Ca2+ signals change dynamically across sleep and wake and after sleep loss using in vivo imaging methods. Synchrony of astroglial Ca2+ signals decreases during sleep compared to wake and after sleep deprivation. STIM1 regulation of astroglial Ca2+ plays a role in the normal compensatory response to sleep loss.
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