Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles.
Methods of Generating Dielectrophoretic Force for Microfluidic Manipulation of Bioparticles.
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DOI：
10.1021/acsbiomaterials.1c00083
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发表时间：
2021-06-14
影响因子：
5.8
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
Kwizera EA;Sun M;White AM;Li J;He X
对包括活细胞在内的微尺度生物颗粒的操纵在生物工程和生物技术领域具有重要意义。介电电泳（DEP）是介电粒子与电场之间的相互作用，是生物粒子分离、分选和捕获等领域中应用最广泛的技术之一。如果生物颗粒在电场强度和/或电场相位不均匀的电场中具有与周围介质不同的极化，则生物颗粒经历DEP力。一个全面的文献调查表明，DEP为基础的微流体装置用于操纵生物颗粒可以分类的方法，通过图案化的微通道，电极和介质产生的DEP力的非均匀性。这些方法连同DEP力产生的理论在这篇综述中进行了描述，提供了一个总结的方法和材料，已被用来操纵各种生物颗粒的各种特定的生物学结果。基于DEP的技术的进一步发展包括识别比当前流行的材料（硅树脂/玻璃）更好地与电极集成的材料，以及通过将其与处理生物颗粒的其他方法相结合来改善生物颗粒的DEP操纵的性能。总的来说，基于DEP的生物颗粒的微流体操纵在各种生物医学应用中具有巨大的潜力。
Manipulation of microscale bioparticles including living cells is of great significance to the broad bioengineering and biotechnology fields. Dielectrophoresis (DEP), which is defined as the interactions between dielectric particles and electric field, is one of the most widely used techniques for the manipulation of bioparticles including cell separation, sorting, and trapping. Bioparticles experience a DEP force if they have a different polarization from the surrounding media in an electric field that is nonuniform in terms of the intensity and/or phase of the electric field. A comprehensive literature survey shows that the DEP-based microfluidic devices for manipulating bioparticles can be categorized according to the methods of creating the nonuniformity via patterned microchannels, electrodes, and media to generate the DEP force. These methods together with the theory of DEP force generation are described in this review, to provide a summary of the methods and materials that have been used to manipulate various bioparticles for various specific biological outcomes. Further developments of DEP-based technologies include identifying materials that better integrate with electrodes than current popular materials (silicone/glass) and improving the performance of DEP manipulation of bioparticles by combining it with other methods of handling bioparticles. Collectively, DEP-based microfluidic manipulation of bioparticles holds great potential for various biomedical applications.
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3D打印的微流体。
DOI：
10.1002/anie.201504382
发表时间：
2016-03-14
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Au AK;Huynh W;Horowitz LF;Folch A
通讯作者：
Folch A
影响因子：
6.1
作者：
Bazant, Martin Z.;Ben, Yuxing
通讯作者：
Ben, Yuxing
影响因子：
6.1
作者：
Barbulovic-Nad, I;Xuan, XC;Li, DQ
通讯作者：
Li, DQ
影响因子：
1.6
作者：
Blaire, Guillaume;Masse, Alain;Reyne, Gilbert
通讯作者：
Reyne, Gilbert
影响因子：
6.1
作者：
Baret, Jean-Christophe;Miller, Oliver J.;Griffiths, Andrew D.
通讯作者：
Griffiths, Andrew D.